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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine

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Author(s):
Siqueira das Neves, Lais Mara ; Marcondes Ferreira Leite, Gabriella de Paula ; Marcolino, Alexandre Marcio ; Pinfildi, Carlos Eduardo ; Garcia, Sergio Britto ; de Araujo, Joao Eduardo ; de Oliveira Guirro, Elaine Caldeira
Total Authors: 7
Document type: Journal article
Source: Lasers in Medical Science; v. 32, n. 2, p. 335-341, FEB 2017.
Web of Science Citations: 8
Abstract

The aim of this study was to investigate the effect of laser photobiomodulation (PBM) on the viability of the transverse rectus abdominis musculocutaneous (TRAM) flap in rats subjected to the action of nicotine. We evaluated 60 albino Wistar rats, divided into six groups of ten animals. Group 1 (saline) underwent the surgical technique to obtain a TRAM flap; group 2 (laser 830 nm) underwent the surgical technique and was irradiated with a laser 830 nm; group 3 (laser 660 nm) underwent the surgical technique and was irradiated with a laser 660 nm; group 4 was treated with nicotine subcutaneously (2 mg/kg/2x/day/4 weeks) and underwent surgery; group 5 (nicotine + laser 830 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 830 nm; group 6 (nicotine + laser 660 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 660 nm. The application of PBM occurred immediately after surgery and on the two following days. The percentage of necrosis was assessed using the AxioVisionA (R) software. The number of mast cells (toluidine blue staining) was evaluated, and immunohistochemistry was performed to detect vascular endothelial growth factor expression (anti-VEGF-A), fibroblasts (anti-basic FGF), and neoformed vessels (anti-CD34). PBM with a wavelength of 830 nm increased the viability of the TRAM flap, with a smaller area of necrosis, increased number of mast cells, and higher expression of VEGF and CD34. PBM increases the viability of musculocutaneous flaps treated with to nicotine. (AU)

FAPESP's process: 13/07732-7 - Low level laser and high voltage electrical stimulation on the viability of flap musculafasciocutaneous transverse rectus abdominis in rats subjected to the action of nicotine
Grantee:Elaine Caldeira de Oliveira Guirro
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07602-6 - Low level laser and High Volt Electrical Stimulation on viability of flap musculofasciocutanneous transverse rectus abdominis in rats submitted to action of nicotine.
Grantee:Lais Mara Siqueira das Neves
Support Opportunities: Scholarships in Brazil - Doctorate