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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

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Silva, Edmundo ; Reis de Vasconcellos, Luana Marotta ; Rodrigues, Bruno V. M. ; dos Santos, Danilo Martins ; Campana-Filho, Sergio P. ; Marciano, Fernanda Roberta ; Webster, Thomas J. ; Lobo, Anderson Oliveira
Total Authors: 8
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 73, p. 31-39, APR 1 2017.
Web of Science Citations: 12

Herein, we developed honeycomb-like scaffolds by combining poly (D, L-lactic acid) (PDLLA) with a high amount of graphene/multi-walled carbon nanotube oxides (MWCNTO-GO, 50% w/w). From pristine multi-walled carbon nanotubes (MWCNT) powders, we produced MWCNTO-GO via oxygen plasma etching (OPE), which promoted their exfoliation and oxidation. Initially, we evaluated PDLLA and PDLLA/MWCNTO-GO scaffolds for tensile strength tests, cell adhesion and cell viability (with osteoblast-like MG-63 cells), alkaline phosphatase (ALP, a marker of osteoblast differentiation) activity and mineralized nodule formation. In vivo tests were carried out using PDLLA and PDLLA/MWCNTO-GO scaffolds as fillers for critical defects in the tibia of rats. MWCNTO-GO loading was responsible for decreasing the tensile strength and elongation-at-break of PDLLA scaffolds, although the high mechanical performance observed (similar to 600 MPa) assures their application in bone tissue regeneration. In vitro results showed that the scaffolds were not cytotoxic and allowed for osteoblast-like cell interactions and the formation of mineralized matrix nodules. Furthermore, MG-63 cells grown on PDLIA/MWCNTO-GO significantly enhanced osteoblast ALP activity compared to controls (cells alone), while the PDLLA group showed similar ALP activity when compared to controls and PDLLA/MWCNTO-GO. Most impressively, in vivo tests suggested that compared to PDLLA scaffolds, PDLLA/MWCNTO-GO had a superior influence on bone cell activity, promoting greater new bone formation. In summary, the results of this study highlighted that this novel scaffold (MWCNTO-GO, 50% w/w) is a promising alternative for bone tissue regeneration and, thus, should be further studied. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 15/09697-0 - Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Scholarships abroad - Research
FAPESP's process: 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers
Grantee:Bruno Vinícius Manzolli Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/00575-1 - Functional biomembranes based on peptides and bottlebrush polymers to tissue engineering
Grantee:Fernanda Roberta Marciano
Support Opportunities: Scholarships abroad - Research