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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Toll-Like Receptor 2 Is Required for Inflammatory Process Development during Leishmania infantum Infection

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Sacramento, Lais A. ; da Costa, Jessica L. ; de Lima, Mikhael H. F. ; Sampaio, Pedro A. ; Almeida, Roque P. ; Cunha, Fernando Q. ; Silva, Joao S. ; Carregaro, Vanessa
Total Authors: 8
Document type: Journal article
Web of Science Citations: 9

Visceral leishmaniasis (VL) is a chronic and fatal disease caused by Leishmania infantum in Brazil. Leukocyte recruitment to infected tissue is a crucial event for the control of infections such as VL. Among inflammatory cells, neutrophils are recruited to the site of Leishmania infection, and these cells may control parasite replication through oxidative or non-oxidative mechanisms. The recruitment, activation and functions of the neutrophils are coordinated by pro-inflammatory cytokines and chemokines during recognition of the parasite by pattern recognition receptors (PRRs). Here, we demonstrated that the Toll-like receptor 2 (TLR2) signaling pathway contributes to the development of the innate immune response during L. infantum infection. The protective mechanism is related to the appropriate recruitment of neutrophils to the inflammatory site. Neutrophil migration is coordinated by DCs that produce CXCL1 and provide a prototypal Th1 and Th17 environment when activated via TLR2. Furthermore, infected TLR2(-/-) mice failed to induce nitric oxide synthase (iNOS) expression in neutrophils but not in macrophages. In vitro, infected TLR2(-/-) neutrophils presented deficient iNOS expression, nitric oxide (NO) and TNF-alpha production, decreased expression of CD11b and reduced L. infantum uptake capacity. The non-responsive state of neutrophils is associated with increased amounts of IL-10. Taken together, these data clarify new mechanisms by which TLR2 functions in promoting the development of the adaptive immune response and effector mechanisms of neutrophils during L. infantum infection. (AU)

FAPESP's process: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases
Grantee:Fernando de Queiroz Cunha
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/12526-2 - Regulatory mechanisms of inflammatory responses in the severity of visceral leishmaniasis
Grantee:Vanessa Carregaro Pereira
Support type: Regular Research Grants