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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigation of biological interactions of chitosan-collagen-hydroxyapatite composite with bone tissue in sheep

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Marcondes, G. M. ; Nobrega, F. S. ; Correa, L. ; Arana-Chavez, V. E. ; Plepis, A. M. G. ; Martins, V. C. A. ; Zoppa, A. L. V.
Total Authors: 7
Document type: Journal article
Source: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 68, n. 6, p. 1531-1538, NOV-DEC 2016.
Web of Science Citations: 1

ABSTRACT Fracture management poses a great challenge to large animal practitioners. Osteosynthesis alone is often insufficient to provide satisfactory outcomes in large animals; therefore, several research efforts have been made to investigate and develop bone substitutes capable of promoting bone regeneration. Chitosan-collagen-hydroxyapatite composites constitute a promising alternative given their similar composition to bone. Six Santa Inês ewes were submitted to the creation of experimental 7mm wide unicortical defects on the dorsomedial aspect of the proximal III/IV metacarpal bone diaphysis. Limbs were randomly selected for treatment with chitosan-collagen-hydroxyapatite composite or to serve as untreated controls. Biopsy fragments were collected from the bone/new bone or the bone/biomaterial interface (control and treated defects respectively) within 60 days of surgery; composite biocompatibility was assessed using light and scanning electron microscopy. Histomorphometric analysis under light microscopy revealed greater percentage of new bone tissue in control compared to treated defects (80% and 63.5% respectively; P<0.05). No scar tissue formation, foreign body or chronic inflammatory reactions were observed. Scanning electron microscopy revealed invasion of the composite by new bone tissue. The chitosan-collagen-hydroxyapatite composite studied is biocompatible with bone and shows osteoconductive properties in sheep. (AU)

FAPESP's process: 12/23629-9 - Use of composite (plaid bone) the basis of chitosan, collagen and hydroxyapatite as a bone substitute in bone defects induced experimentally in iii and IV metacarpal bones of sheep: histological and biomechanical evaluations
Grantee:André Luis do Valle de Zoppa
Support Opportunities: Regular Research Grants