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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Using the Amino Acid Network to Modulate the Hydrolytic Activity of beta-Glycosidases

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Tamaki, Fabio K. ; Souza, Diorge P. ; Souza, Valquiria P. ; Ikegami, Cecilia M. ; Farah, Chuck S. ; Marana, Sandro R.
Total Authors: 6
Document type: Journal article
Source: PLoS One; v. 11, n. 12 DEC 9 2016.
Web of Science Citations: 1

The active site residues in GH1 beta-glycosidases are compartmentalized into 3 functional regions, involved in catalysis or binding of glycone and aglycone motifs from substrate. However, it still remains unclear how residues outside the active site modulate the enzymatic activity. To tackle this question, we solved the crystal structure of the GH1 beta-glycosidase from Spodoptera frugiperda (Sf beta gly) to systematically map its residue contact network and correlate effects of mutations within and outside the active site. External mutations neighbouring the functional residues involved in catalysis and glycone-binding are deleterious, whereas mutations neighbouring the aglycone-binding site are less detrimental or even beneficial. The large dataset of new and previously characterized Sfpgly mutants supports that external perturbations are coherently transmitted to active site residues possibly through contacts and specifically disturb functional regions they interact to, reproducing the effects observed for direct mutations of functional residues. This allowed us to suggest that positions related to the aglycone-binding site are preferential targets for introduction of mutations aiming to further improve the hydrolytic activity of beta-glycosidases. (AU)

FAPESP's process: 08/55914-9 - Development of beta-glycosidases designed to improve the efficiency of noncomplexed cellulase systems
Grantee:Sandro Roberto Marana
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/19439-5 - Structural networks and functional properties in enzymes
Grantee:Sandro Roberto Marana
Support Opportunities: Regular Research Grants