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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Apocynin reduces blood pressure and restores the proper function of vascular endothelium in SHR

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Perassa, Ligia A. ; Graton, Murilo E. ; Potje, Simone R. ; Troiano, Jessica A. ; Lima, Mariana S. ; Vale, Gabriel T. ; Pereira, Ariana A. F. ; Nakamune, Ana Claudia M. S. ; Sumida, Doris H. ; Tirapelli, Carlos R. ; Bendhack, Lusiane M. ; Antoniali, Cristina
Total Authors: 12
Document type: Journal article
Source: VASCULAR PHARMACOLOGY; v. 87, p. 38-48, DEC 2016.
Web of Science Citations: 7
Abstract

This study has evaluated how the vascular endothelium of hypertensive rats chronically treated with apocynin affects acetylcholine (ACh), sodium nitroprusside (SNP), and phenylephrine (PE) action on the nitric oxide (NO) signal transduction pathway in endothelial (EC) and vascular smooth muscle cells. Treatment with apocynin significantly reduced the mean arterial pressure in spontaneously hypertensive rats (SHR). In addition, apocynin improved the impaired ACh hypotensive effect on SHR. Although systemic oxidative stress was high in SHR, SHR treated with apocynin and normotensive rats presented similar systemic oxidative stress levels. Endothelium significantly blunted PE contractions in intact aortas of treated SHR. The ACh effect was impaired in resistance arteries and aortas of SHR, but this same effect was improved in treated SHR. The SNP potency was higher in intact resistance arteries of treated SHR than in intact resistance arteries of untreated SHR. NO and calcium concentrations increased, whereas reactive oxygen species levels decreased in EC of treated SHR. Aortas of untreated and treated SHR did not differ in terms of sGC alpha or beta units expression. Aorta of treated SHR expressed higher eNOS levels as compared to aorta of untreated SHR. The study groups did not differ with respect to NOX1, NOXO1, or NOX4 expression. However, treatment with apocynin normalized overexpression of NOX2 and its subunit p47phox in aortas of SHR. Based on all the results presented in this study, we suggest apocynin increases NO biovailability by different mechanisms, restoring the proper function of vascular endothelium in SHR. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 12/01733-9 - Analysis of the effect of apocynin on the hipotensive responses induced by no donors in spontaneously hypertensive rats (SHR)
Grantee:Murilo Eduardo Graton
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/04619-0 - Analysis in vivo and in vitro effect of apocynin on endothelial dysfunction in spontaneously hypertensive rats (SHR).
Grantee:Ligia Arnedo Perassa
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/20998-0 - Analysis in vivo and in vitro effect of apocynin on endothelial dysfunction in spontaneously hypertensive rats (SHR)
Grantee:Cristina Antoniali Silva
Support type: Regular Research Grants