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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

AHNAK enables mammary carcinoma cells to produce extracellular vesicles that increase neighboring fibroblast cell motility

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Silva, Thaiomara A. ; Smuczek, Basilio ; Valadao, Iuri C. ; Dzik, Luciana M. ; Iglesia, Rebeca P. ; Cruz, Mario C. ; Zelanis, Andre ; de Siqueira, Adriane S. ; Serrano, Solange M. T. ; Goldberg, Gary S. ; Jaeger, Ruy G. ; Freitas, Vanessa M.
Total Authors: 12
Document type: Journal article
Source: ONCOTARGET; v. 7, n. 31, p. 49998-50016, AUG 2 2016.
Web of Science Citations: 13

Extracellular vesicles play important roles in tumor development. Many components of these structures, including microvesicles and exosomes, have been defined. However, mechanisms by which extracellular vesicles affect tumor progression are not fully understood. Here, we investigated vesicular communication between mammary carcinoma cells and neighboring nontransformed mammary fibroblasts. Nonbiased proteomic analysis found that over 1% of the entire proteome is represented in these vesicles, with the neuroblast differentiation associated protein AHNAK and annexin A2 being the most abundant. In particular, AHNAK was found to be the most prominent component of these vesicles based on peptide number, and appeared necessary for their formation. In addition, we report here that carcinoma cells produce vesicles that promote the migration of recipient fibroblasts. These data suggest that AHNAK enables mammary carcinoma cells to produce and release extracellular vesicles that cause disruption of the stroma by surrounding fibroblasts. This paradigm reveals fundamental mechanisms by which vesicular communication between carcinoma cells and stromal cells can promote cancer progression in the tumor microenvironment. (AU)

FAPESP's process: 11/09472-7 - AHNAK regulates formation and exchange of extracellular vesicles between breast tumor cells and fibroblasts
Grantee:Thaiomara Alves Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/07699-1 - Protease ADAMTS1 influencing breast cancer behavior and microenvironment
Grantee:Vanessa Morais Freitas
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/00544-6 - Signaling components that travel between and transformed and nontransformed cells by microvesicles
Grantee:Vanessa Morais Freitas
Support type: Research Grants - Visiting Researcher Grant - International
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC