Mammalian cell entry (Mce) protein of Leptospira i... - BV FAPESP
Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mammalian cell entry (Mce) protein of Leptospira interrogans binds extracellular matrix components, plasminogen and 2 integrin

Full text
Author(s):
Cosate, Maria Raquel ; Siqueira, Gabriela Hase ; de Souza, Gisele Oliveira ; Vasconcellos, Silvio Arruda ; Nascimento, Ana Lucia T. O.
Total Authors: 5
Document type: Journal article
Source: MICROBIOLOGY AND IMMUNOLOGY; v. 60, n. 9, p. 586-598, SEP 2016.
Web of Science Citations: 3
Abstract

A severe re-emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus Leptospira. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and invasion through the hosts. It has been shown that Mce of pathogenic Leptospira spp. is an RGD (Arg-Gly-Asp)-motif-dependent virulence factor, responsible for infection of cells and animals. In the present article, we decided to further study the repertoire of the Mce activities in leptospiral biological properties. We report that the recombinant Mce is a broad-spectrum ECM-binding protein, capable of interacting with laminin, cellular and plasma fibronectin and collagen IV. Dose-response interaction was observed for all the components, fulfilling ligand-receptor requirements. Mce is a PLG binding protein capable to recruit this component from NHS, generating PLA in the presence of PLG activator. Binding of Mce was also observed with the leukocyte cell receptors L2 {[}(CD11a/CD18)-LFA-1] and M2 {[}(CD11b/CD18)-Mac-1], suggesting the involvement of this protein in the host immune response. Indeed, virulent Leptospira L1-130 was capable of binding both integrins, whereas culture-attenuated M-20 strain only bind to M2 {[}(CD11b/CD18)-Mac-1]. To the best of our knowledge, this is the first work to describe that Mce surface protein could mediate the attachment of Leptospira interrogans to human cell receptors L2(CD11a/CD18) and M2(CD11b/CD18). (AU)

FAPESP's process: 14/50981-0 - Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis
Grantee:Ana Lucia Tabet Oller do Nascimento
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/23913-9 - Indentification and characterization of proteins of Leptospira interrogans involved in host-pathogen interactions
Grantee:Ana Lucia Tabet Oller do Nascimento
Support Opportunities: Regular Research Grants
FAPESP's process: 14/03792-8 - "Evaluation of the immune evasion by pathogenic Leptospira interrogans at the terminal complement system pathway level"
Grantee:Gabriela Hase Siqueira
Support Opportunities: Scholarships in Brazil - Post-Doctoral