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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gingival crevicular fluid levels of protease-activated receptors type 1 and type 2 in diabetic patients with periodontitis

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Abreu, I. S. ; Euzebio Alves, V. T. ; Benedete, A. P. S. ; Bueno da Silva, H. A. ; Franca, B. N. ; Saraiva, L. ; Lima, L. A. ; Carvalho, M. H. ; Holzhausen, M.
Total Authors: 9
Document type: Journal article
Source: JOURNAL OF PERIODONTAL RESEARCH; v. 51, n. 5, p. 577-585, OCT 2016.
Web of Science Citations: 0

Background and Objective: Protease activated receptor type 1 (PAR1) seems to play a role in periodontal repair, while PAR2 is associated with periodontal inflammation. As diabetes is a known risk factor for periodontal disease, the aim of this study was to evaluate the influence of type 2 diabetes on PAR1 and PAR2 mRNA expression in the gingival crevicular fluid of patients with chronic periodontitis before and after non-surgical periodontal treatment. Material and Methods: Gingival crevicular fluid samples and clinical parameters consisting of measuring probing depth, clinical attachment level, bleeding on probing and plaque index were collected from systemically healthy patients and patients with type 2 diabetes and chronic periodontitis, at baseline and after non-surgical periodontal therapy. PAR1 and PAR2, as well as the presence of the proteases RgpB gingipain and neutrophil proteinase-3 were assessed by quantitative polymerase chain reaction in the gingival crevicular fluid. Results: The periodontal clinical parameters significantly improved after periodontal therapy (p < 0.01). Diabetes led to increased expression of PAR1 in gingival crevicular fluid, and in the presence of chronic periodontitis, it significantly decreased the expression of PAR1 and PAR2 (p < 0.05). Moreover, non-surgical periodontal treatment in diabetics resulted in increased expression of PAR1 and PAR2 (p < 0.05), and decreased expression of RgpB gingipain and proteinase-3 (p < 0.05). Conclusion: The present data demonstrated that diabetes was associated with an altered expression of PAR1 and PAR2 in the gingival crevicular fluid cells of subjects with chronic periodontitis. Future studies are necessary to elucidate the effects of PAR1 upregulation in periodontally healthy sites and PAR2 downregulation in chronic periodontitis sites on the increased susceptibility and severity of periodontitis in diabetes. (AU)

FAPESP's process: 09/16373-5 - Oxidative stress markers evaluation before and after periodontal treatment of Diabetics type 2 patients with generalized chronic periodontitis and healthy periodontium
Grantee:Luiz Antonio Pugliesi Alves de Lima
Support Opportunities: Regular Research Grants
FAPESP's process: 10/20477-8 - Evaluation of the protease activated receptor 2(PAR-2) expression in chronic periodontitis patients, before and after non-surgical periodontal treatment
Grantee:Vanessa Tubero Euzebio Alves
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/06387-7 - Expression of protease activated receptor 2 (PAR-2) in crevicular fluid cells of type 2-diabetic patients
Grantee:Bruno Nunes de França
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/16605-0 - Effect of periodontal therapy on protease activated receptor 2 (PAR-2) expression in chronic periodontitis patients.
Grantee:Marinella Holzhausen Caldeira
Support Opportunities: Regular Research Grants