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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Extracellular vesicles from Paracoccidioides brasiliensis induced M1 polarization in vitro

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da Silva, Thiago Aparecido ; Roque-Barreira, Maria Cristina ; Casadevall, Arturo ; Almeida, Fausto
Total Authors: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 6, OCT 24 2016.
Web of Science Citations: 14

Extracellular vesicles (EVs) released by eukaryotes, archaea, and bacteria contain proteins, lipids, polysaccharides, and other molecules. The cargo analysis of EVs shows that they contain virulence factors suggesting a role in the pathogenesis of infection. The proteome, lipidome, RNA content, and carbohydrate composition of EVs from Paracoccidioides brasiliensis and Paracoccidioides lutzii were characterized. However, the effects of P. brasiliensis EVs on the host immune system have not yet been investigated. Herein, we verified that EVs from P. brasiliensis induce the production of proinflammatory mediators by murine macrophages in a dose-dependent manner. Addition of EV to macrophages also promoted transcription of the M1-polarization marker iNOs and diminish that of the M2 markers Arginase-1, Ym-1, and FIZZ-1. Furthermore, the augmented expression of M2-polarization markers, stimulated by IL-4 plus IL-10, was reverted toward an M1 phenotype in response to secondary stimulation with EVs from P. brasiliensis. The ability of EVs from P. brasiliensis to promote M1 polarization macrophages favoring an enhanced fungicidal activity, demonstrated by the decreased CFU recovery of internalized yeasts, with comparable phagocytic efficacy. Our results suggest that EVs from P. brasiliensis can modulate the innate immune response and affect the relationship between P. brasiliensis and host immune cells. (AU)

FAPESP's process: 16/03322-7 - Role of galectin-3 in the Cryptococcus neoformans infection
Grantee:Fausto Bruno dos Reis Almeida
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/04088-0 - Lectin from pathogens
Grantee:Maria Cristina Roque Antunes Barreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/04877-2 - Design of new therapeutic strategies, based on the carbohydrate recognition, against cryptococcosis
Grantee:Thiago Aparecido da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/10741-8 - Effect of N-glycosylation inhibition on the transcriptional and proteomic profiles of Paracoccidioides brasiliensis
Grantee:Fausto Bruno dos Reis Almeida
Support type: Scholarships in Brazil - Post-Doctorate