Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis

Full text
Author(s):
Galeano, E. ; Barroso, A. A. M. ; Vasconcelos, T. S. ; Lopez-Rubio, A. ; Albrecht, A. J. P. ; Victoria Filho, R. ; Carrer, H.
Total Authors: 7
Document type: Journal article
Source: Genetics and Molecular Research; v. 15, n. 3 2016.
Web of Science Citations: 0
Abstract

Weed resistance to herbicides is a natural phenomenon that exerts selection on individuals in a population. In Brazil, glyphosate resistance was recently detected in Digitaria insularis. The objective of this study was to elucidate mechanisms of weed resistance in this plant, including genetic variability, allelism, amino acid substitutions, gene expression, and enzymatic activity levels. Most of these have not previously been studied in this species. D. insularis DNA sequences were used to analyze genetic variability. cDNA from resistant and susceptible plants was used to identify mutations, alleles, and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) expression, using real-time quantitative reverse transcription-polymerase chain reaction. In addition, EPSPS activity was measured. We found a decrease in genetic variability between populations related to glyphosate application. Substitutions from proline to threonine and tyrosine to cysteine led to a decrease in EPSPS affinity for the glyphosate. In addition, the EPSPS enzymatic activity was slightly higher in resistant plants, whereas EPSPS gene expression was almost identical in both biotypes, suggesting feedback regulation at different levels. To conclude, our results suggest new molecular mechanisms used by D. insularis to increase glyphosate resistance. (AU)

FAPESP's process: 12/10371-3 - BEHAVIOR OF RR SOYBEANS IN DIFFERENT PRODUCTION ENVIRONMENTS, SUBMITTED TO DIFFERENT MANAGEMENTS, FORMULATIONS AND RATES OF GLYPHOSATE
Grantee:Alfredo Junior Paiola Albrecht
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/06299-8 - Transcriptional profile analysis of lignified tissues in Tectona grandis L. f
Grantee:Esteban Galeano Gómez
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 12/10372-0 - Leaf surface and genetic characterization of Digitaria insularis (L.) Fedde and control efficacy of herbicides associations
Grantee:Arthur Arrobas Martins Barroso
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/19294-6 - CAD gene family characterization involved in wood formation tissues in Tectona grandis
Grantee:Tarcísio Sales Vasconcelos
Support type: Scholarships in Brazil - Scientific Initiation