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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries

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Moreira, Monique Rodrigues ; Souza, Ariana Borges ; Soares, Sandra ; Bianchi, Thamires Chiquini ; Eugenio, Daniele de Souza ; Lemes, Danieli Cristina ; Gomes Martins, Carlos Henrique ; Moraes, Thais da Silva ; Tavares, Denise Crispim ; Ferreira, Natalia Helen ; Ambrosio, Sergio Ricardo ; Sola Veneziani, Rodrigo Cassio
Total Authors: 12
Document type: Journal article
Source: Fitoterapia; v. 112, p. 211-216, JUL 2016.
Web of Science Citations: 11

Many studies have reported that medicinal plant extracts can inhibit oral pathogen growth or adhesion to surfaces and therefore reduce dental caries formation. The addition of these extracts to oral products like mouthwashes and dentifrices is considered an important strategy in caries control. In this sense, we have developed a Mikania glomerata extract with high ent-kaurenoic acid content (KAMg). So, this work describes the preparation of such extract and the development of a validated HPLC-DAD method to determine its ent-kaurenoic acid (KA) content. Herein it is also described the KAMg in vitro antibacterial evaluation against several cariogenic bacteria in comparison with KA and the investigation of further aspects of the KAMg activity. Toxicological aspects of the developed extract were evaluated by assessing its cytotoxicity and genotoxicity. KA and a KA-rich extract like KAMg showed to inhibit the growth of microorganisms responsible for dental caries at relatively low MIC (Minimum inhibitory concentration) values, albeit not as low as the MIC value obtained for chlorhexidine digluconate (CHD), the golden anticariogenic standard approved by the American Dental Association Council on Dental Therapeutics. However, KAMg was more effective to inhibit the formation of a Streptococcus mutans biofilm with four times lower MICB50 (minimum inhibitory concentration that reduces 50% of the biofilm) value as compared with CHD. Taldng into account all these data and considering the absence of genotoxic and cytotoxic activity under the tested conditions, it is suggested that KAMg is a natural product to be considered as active ingredient in oral care products. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/17700-0 - Salvia officinalis: chemical constituents and anticariogenic potential evaluation
Grantee:Monique Rodrigues Moreira
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support Opportunities: Research Projects - Thematic Grants