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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of Molecularly Imprinted Polymers for Solid Phase Extraction of Parabens in Plasma Samples and Analysis by UHPLC-MS/MS

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Author(s):
Mariane V. Roldão [1] ; Lidervan P. Melo [2] ; Luis Felippe C. Miranda [3] ; Marcos G. Resende [4] ; Maria Eugênia C. Queiroz [5]
Total Authors: 5
Affiliation:
[1] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil
[2] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil
[3] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil
[4] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil
[5] Universidade de São Paulo. Faculdade de Filosofia Ciência e Letras de Ribeirão Preto. Departamento de Química - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 28, n. 2, p. 257-265, 2017-02-00.
Abstract

Two molecularly imprinted polymers (MIPs) were synthesized, by sol-gel and radical polymerization procedures. These MIPs were evaluated as selective stationary phases for miniaturized solid phase extraction (MISPE) to determine parabens in plasma samples by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Selective cavities of the MIPs, based on molecular recognition (hydrogen bonds), were revealed after removal of the template (benzylparaben). Both MIPs were characterized by scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). MIP prepared by radical process presented higher selective binding capacity for the target parabens than MIP prepared by sol-gel. Several variables that influence MISPE performance, including sample pH, sorbent washing, elution solvent, and elution solvent volume were also evaluated. According to the analytical validation parameters, the MISPE/UHPLC-MS/MS method is suitable for the analysis of parabens in plasma samples. The effectiveness of this method was demonstrated through the analyses of the umbilical plasma samples from postpartum women. (AU)

FAPESP's process: 12/21178-0 - Development of molecularly imprinted extraction phase for analysis of parabens in plasma samples by liquid chromatography coupled to mass spectrometry sequence
Grantee:Mariane Valéria Roldão
Support type: Scholarships in Brazil - Scientific Initiation