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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses

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Gabriella Dias Arena [1] ; Pedro Luis Ramos-González ; Maria Andréia Nunes [3] ; Camila Chabi Jesus ; Renata Faier Calegario [5] ; Elliot Watanabe Kitajima [6] ; Valdenice Moreira Novelli [7] ; Juliana Freitas-Astúa
Total Authors: 8
[1] Agronomic Institute/Sylvio Moreira Citrus Research Center - Brasil
[3] Agronomic Institute/Sylvio Moreira Citrus Research Center - Brasil
[5] Federal University of Paraná - Brasil
[6] University of São Paulo/ESALQ. Dept. of Plant Pathology and Nematology - Brasil
[7] Agronomic Institute/Sylvio Moreira Citrus Research Center - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Scientia Agricola; v. 74, n. 1, p. 85-89, 2017-02-00.

ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. (AU)

FAPESP's process: 12/18771-0 - Gene expression analysis of model plant in response to citrus leprosis
Grantee:Gabriella Dias Arena
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/00366-8 - Arabidopsis thaliana e Citrus leprosis virus-C: a model for the plant-pathogen interaction study
Grantee:Gabriella Dias Arena
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite
Grantee:Elliot Watanabe Kitajima
Support Opportunities: Research Projects - Thematic Grants