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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of hypoxia inducible factor targeting pharmacological drugs as antileishmanial agents

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Pelegrini, Marina Dal'Bo [1] ; Pereira, Juliana Biar [1] ; Costa, Solange dos Santos [1] ; Salazar Terreros, Myriam Janeth [1] ; Degrossoli, Adriana [1] ; Giorgio, Selma [1]
Total Authors: 6
[1] Univ Estadual Campinas, Inst Biol, Dept Anim Biol, Rua Monteiro Lobato, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE; v. 9, n. 7, p. 633-638, JUL 2016.
Web of Science Citations: 2

Objective: To evaluate whether hypoxia inducible factor (HIF-1 alpha) targeting pharmacological drugs, echinomycin, resveratrol and CdCl2, which inhibit HIF-1 alpha stimulation, and mimosine, which enhances the stability of HIF-1 alpha present antileishmanial properties. Methods: The leishmanicidal effect of drugs was evaluated in mouse macrophages and Balb/c mouse model for cutaneous leishmaniosis. Results: Resveratrol and CdCl2, reduced the parasite load {[}IC50, (27.3 +/- 2.25) mu M and (24.8 +/- 0.95) mu M, respectively]. The IC50 value of echinomycin was (22.7 +/- 7.36) mu M and mimosine did not alter the parasite load in primary macrophages. The macrophage viability IC50 values for resveratrol, echinomycin and CdCl2, and mimosine were >40 mu M, >100 mu M > 200 mu M and>2 000 mu M, respectively. In vivo no differences between cutaneous lesions from control, resveratrol- and echinomycin-treated Balb/c mice were detected. Conclusions: Resveratrol, echinomycin and CdCl2, reduce parasite survival in vitro. The HIF-1 alpha targeting pharmacological drugs require further study to more fully determine their anti-Leishmania potential and their role in therapeutic strategies. (AU)

FAPESP's process: 09/10771-9 - Evaluation and manipulation of oxygen tension in cellular and tissular microenvironments during infectious processes
Grantee:Selma Giorgio
Support Opportunities: Regular Research Grants