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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Global proteome profiling of dental cementum under experimentally-induced apposition

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Author(s):
Salmon, Cristiane R. [1] ; Giorgetti, Ana Paula O. [1] ; Paes Leme, Adriana Franco [2] ; Domingues, Romenia R. [2] ; Sallum, Enilson Antonio [1] ; Alves, Marcelo C. [3] ; Kolli, Tamara N. [4] ; Foster, Brian L. [4] ; Nociti, Jr., Francisco H. [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Div Periodont, Dept Prosthodont & Periodont, Sao Paulo - Brazil
[2] Natl Biosci Lab, Brazilian Synchrotron Light Lab, Campinas, SP - Brazil
[3] ESALQ Univ Sao Paulo, Tech Sect Informat, Piracicaba, SP - Brazil
[4] Ohio State Univ, Coll Dent, Biosci Div, Columbus, OH 43210 - USA
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF PROTEOMICS; v. 141, p. 12-23, JUN 1 2016.
Web of Science Citations: 8
Abstract

Dental cementum (DC) covers the tooth root and has important functions in tooth attachment and position. DC can be lost to disease, and regeneration is currently unpredictable due to limited understanding of DC formation. This study used a model of experimentally-induced apposition (EIA) in mice to identify proteins associated with new DC formation. Mandibular first molars were induced to super-erupt for 6 and 21 days after extracting opposing maxillary molars. Decalcified and formalin-fixed paraffin-embedded mandible sections were prepared for laser capture microdissection. Microdissected protein extracts were analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and the data submitted to repeated measure ANOVA test (RM-ANOVA, alpha = 5%). A total of 519 proteins were identified, with 97 (18.6%) proteins found exclusively in EIA sites and 50 (9.6%) proteins exclusively expressed in control sites. Fifty six (10.7%) proteins were differentially regulated by RM-ANOVA (p < 0.05), with 24 regulated by the exclusive effect of EIA (12 proteins) or the interaction between EIA and time (12 proteins), including serpin la, procollagen C-endopeptidase enhancer, tenascin X (TNX), and asporin (ASPN). In conclusion, proteomic analysis demonstrated significantly altered protein profile in DC under EIA, providing new insights on DC biology and potential candidates for tissue engineering applications. Significance: Dental cementum (DC) is a mineralized tissue that covers the tooth root surface and has important functions in tooth attachment and position. DC and other periodontal tissues can be lost to disease, and regeneration is currently unpredictable due to lack of understanding of DC formation. This study used a model of experimentally-induced apposition (EIA) in mice to promote new cementum formation, followed by laser capture microdissection (LCM) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) proteomic analysis. This approach identified proteins associated with new cementum formation that may be targets for promoting cementum regeneration. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/12486-7 - Proteomic and gene expression comparative analysis of human dental cementum and alveolar bone.
Grantee:Cristiane Ribeiro Salmon
Support Opportunities: Scholarships in Brazil - Post-Doctoral