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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Zinc Chelation Mediates the Lysosomal Disruption without Intracellular ROS Generation

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Matias, Andreza Candido [1] ; Manieri, Tania Maria [1] ; Cerchiaro, Giselle [1]
Total Authors: 3
[1] Fed Univ ABC, UFABC, Ctr Nat Sci & Humanities, Ave Estados 5001, Bloco B, BR-09210170 Santo Andre, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Web of Science Citations: 4

We report the molecular mechanism for zinc depletion caused by TPEN (N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine) in neuroblastoma cells. The activation of p38 MAP kinase and subsequently caspase 3 is not due to or followed by redox imbalance or ROS generation, though these are commonly observed in literature. We found that TPEN is not responsible for ROS generation and the mechanism involves essentially lysosomal disruption caused by intracellular zinc depletion. We also observed a modest activation of Bax and no changes in the Bcl-2 proteins. As a result, we suggest that TPEN causes intracellular zinc depletion which can influence the breakdown of lysosomes and cell death without ROS generation. (AU)

FAPESP's process: 12/01397-9 - Effects of metalloenzyme superoxide dismutase expression in the generation of reactive oxygen species in cultured mammalian cells and in motor neurons
Grantee:Giselle Cerchiaro
Support type: Regular Research Grants