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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

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Bertholim, Luciana [1, 2] ; Zelanis, Andre [3, 2] ; Oliveira, Ana K. [1, 2] ; Serrano, Solange M. T. [2]
Total Authors: 4
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil
[2] Inst Butantan, CeTICS, Lab Especial Toxinol Aplicada, Ctr Toxins Immune Response & Cell Signaling, Av Vital Brasil 1500, BR-05503000 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Ciencia & Tecnol, Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Amino Acids; v. 48, n. 5, p. 1331-1335, MAY 2016.
Web of Science Citations: 2

The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6' sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1' position, followed by hydrophobic residues in P2'. PICS confirmed existing data on prime site specificity of SVMPs. (AU)

FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 11/16623-1 - Proteomic analysis of the proteolytic activity of HF3, a metalloproteinase from the venom of Bothrops jararaca, upon human and snake plasma.
Grantee:Luciana Bertholim Nasciben
Support Opportunities: Scholarships in Brazil - Master