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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biological Evaluation of PLDLA Polymer Synthesized as Construct on Bone Tissue Engineering Application

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Author(s):
Mas, Bruna Antunes [1] ; de Luna Freire, Diego Coutinho [2] ; de Melo Cattani, Silvia Mara [2] ; Motta, Adriana Cristina [2] ; Peris Barbo, Maria Lourdes [2] ; de Rezende Duek, Eliana Aparecida [2, 1]
Total Authors: 6
Affiliation:
[1] State Univ Campinas UNICAMP, Rua Mendeleyev 200, BR-13083970 Campinas, SP - Brazil
[2] PUC SP, Rua Joubert Wey 290, BR-18030070 Sorocaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS; v. 19, n. 2, p. 300-307, MAR-APR 2016.
Web of Science Citations: 2
Abstract

In bone tissue engineering, cell-scaffold constructs are used to stimulate complete, functional tissue replacement that does not occur naturally in critical-size defects. In this report, we describe the application potential of poly (L-co-D,L lactic acid)-PLDLA 70/30, synthesized in house as constructs loaded with osteoblast-like cells on bone tissue engineering. In vitro biological results show that the porogen leached PLDLA scaffolds are cytocompatible with osteoblast cells, able to stimulate significant cells growth during the first 14 days of culture, during which the morphology and cell behavior of osteoblasts cultured on the scaffolds were monitored by scanning electron microscopy (SEM). In vivo, PLDLA constructs were implanted in 5 mm bilateral critical-size defects created in rat-calvariae and then evaluated histologically 8 and 12 weeks after implantation. The histological results showed that PLDLA constructs supported the growth of new tissue, with a degradation rate close to that of native bone formation and decrease of inflammatory response over time of implantation. These data provide evidences that the synthesized PLDLA polymer has application potential as construct for bone tissue engineering. (AU)

FAPESP's process: 12/12081-2 - Collagen imobilization on poly (L-co-D, L lactic acid) scaffolds
Grantee:Eliana Aparecida de Rezende Duek
Support type: Regular Research Grants