Anti-inflammatory and antinociceptive effects of racemic goniothalamin, a styryl lactone

Aims: The present study aimed to further investigate the anti-inflammatory activity of goniothalamin (GTN), a styryl lactone, as well as its antinociceptive effects. Main methods: The anti-inflammatory activity was evaluated in models of paw edema induced by different mediators in mice and carrageenan-induced peritonitis. Evaluation of the antinociceptive effect was performed through acetic acid-induced writhing test and formalin test. Activity of GTN on gene expression levels of interleukin-1beta (IL-1 beta), induced nitric oxidase synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated in vitro in lipopolysaccharide (LPS)-stimulated macrophage (RAW 264.7), as well as gene expression and protein levels of tumor necrosis factor-alpha (TNF-alpha). Key findings: Pretreatment with GIN (300 mg/kg) significantly reduced paw edema induced by compound 48/80, prostaglandin E-2, phospholipase A(2) and bradyldnin. GIN (10,30 and 100 mg/kg) inhibited leukocyte migration in the peritonitis model and gene expression levels of IL-1 beta, iNOS and TNF-alpha, as well as TNF-alpha protein levels, in LPS-stimulated macrophages, without affecting COX-2 gene expression levels. GIN inhibited nociception induced by acetic acid in the writhing model and in the formalin test, when both neurogenic and inflammatory phases were inhibited. Significance: For the first time the acute anti-inflammatory profile of GTN is characterized and its antinociceptive activity reported. The current study shows that GTN inhibits both vascular and cellular phases of inflammation, with bradykinin and PLA(2) induced inflammation being the most affected by GIN. Its anti-inflammatory effects also involved the in vitro inhibition of gene expression of alarm cytokines and mediators as IL-1 beta, iNOS and TNF-alpha. (C) 2015 Elsevier Inc. All rights reserved. (AU)