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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Periapical Lesions Decrease Insulin Signal and Cause Insulin Resistance

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Astolphi, Rafael Dias [1] ; Curbete, Mariane Machado [1] ; Colombo, Natalia Helena [1] ; Shirakashi, Daisy Jaqueline [1] ; Chiba, Fernando Yamamoto [1] ; Carrara Prieto, Annelise Katrine [1] ; Angelo Cintra, Luciano Tavares [1] ; Mogami Bomfim, Suely Regina ; Ervolino, Edilson [1] ; Sumida, Doris Hissako [1]
Total Authors: 10
[1] UNESP Univ Estadual Paulista, Aracatuba Sch Dent, Aracatuba, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 39, n. 5, p. 648-652, MAY 2013.
Web of Science Citations: 18

Introduction: Inflammatory cytokines are associated with decreased insulin signal transduction. Moreover, local oral inflammation, such as that accompanying periodontal disease, is associated with insulin resistance and type 2 diabetes mellitus. The aim of this study was to evaluate the effect of periapical lesions (PLs) on insulin signaling and insulin sensitivity in rats. We hypothesized that PLs alter systemic insulin signaling and insulin sensitivity via elevated plasmatic tumor necrosis factor alpha (TNF-alpha). Methods: Wistar rats were divided into control (CN) and PL groups. PLs were induced by exposing pulpal tissue to the oral environment. After 30 days, insulin sensitivity was measured using the insulin tolerance test. After euthanization, maxillae were processed for histopathology. Plasmatic concentrations of tumor necrosis factor alpha (TNF-alpha) were determined via the enzyme-linked immunosorbent assay. Insulin signal transduction was evaluated using insulin receptor substrate tyrosine phosphorylation status and serine phosphorylation status in periepididymal white adipose tissue via Western blotting. For insulin signaling and insulin tolerance tests, the analyses performed were analysis of variance followed by the Tukey post hoc test. For TNF-alpha analysis, the Student's t test was used. In all tests, P < .05 was considered significant. Results: The rats with PLs showed higher plasmatic TNF-alpha, lower constant rate for glucose disappearance values, and reduced pp185 tyrosine phosphorylation status but no change in serine phosphorylation status in white adipose tissue after insulin stimulation. Conclusions: PLs can cause alterations to both insulin signaling and insulin sensitivity, probably because of elevation of plasmatic TNF-alpha. The results from this study emphasize the importance of the prevention of local inflammatory diseases, such as PLs, with regard to the prevention of insulin resistance. (AU)

FAPESP's process: 11/04255-8 - Evaluation of insulin signal in adult rats with pulp disease
Grantee:Rafael Dias Astolphi
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/13454-4 - Evaluation of the insulin signal in skeletal muscle tissue of adult rats with pulp disease
Grantee:Mariane Machado Curbete
Support type: Scholarships in Brazil - Scientific Initiation