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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

fosI Is a New Integron-Associated Gene Cassette Encoding Reduced Susceptibility to Fosfomycin

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Pelegrino, Karla de Oliveira [1] ; Campos, Juliana Coutinho [2] ; Ferreira Sampaio, Suely Carlos [3] ; Lezirovitz, Karina [4] ; Seco, Bruna Mara [2] ; Pereira, Mayne de Oliveira [2] ; da Costa Rocha, Darlan Augusto [2] ; Jove, Thomas [5] ; Nicodemo, Antonio Carlos [1] ; Mello Sampaio, Jorge Luiz [2, 6]
Total Authors: 10
[1] Univ Sao Paulo, Sch Med, Dept Infect Dis, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Pharm, Clin Anal & Toxicol Dept, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Microbiol & Imunol, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Sch Med, Hosp Clin, Otorhinolaryngol Lab LIM32, Sao Paulo, SP - Brazil
[5] Univ Limoges, INSERM, CHU Limoges, UMR S 1092, Limoges - France
[6] Fleury Med & Hlth, Dept Microbiol, Sao Paulo, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Antimicrobial Agents and Chemotherapy; v. 60, n. 1, p. 686-688, JAN 2016.
Web of Science Citations: 0

In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 mu g/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 mu g/ml when the gene was cloned into the pBC\_SK(-) vector and expressed in E. coli TOP10. (AU)

FAPESP's process: 10/00807-3 - Phenotypic and genotypic characterization of antimicrobial resistance in rapidly growing mycobacteria
Grantee:Jorge Luiz Mello Sampaio
Support Opportunities: Regular Research Grants