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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Saccharification of ozonated sugarcane bagasse using enzymes from Myceliophthora thermophila JCP 1-4 for sugars release and ethanol production

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Author(s):
Pereira, Josiani de Cassia [1] ; Travaini, Rodolfo [2] ; Marques, Natalia Paganini [3] ; Bolado-Rodriguez, Silvia [2] ; Bocchini Martins, Daniela Alonso [3]
Total Authors: 5
Affiliation:
[1] UNESP Univ Estadual Paulista, IBILCE, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Valladolid UVa, Dept Chem Engn & Environm Technol, Calle Doctor Mergelina S-N, Valladolid 47005 - Spain
[3] UNESP Univ Estadual Paulista, IQ, Dept Biochem & Chem Technol, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Bioresource Technology; v. 204, p. 122-129, MAR 2016.
Web of Science Citations: 14
Abstract

The saccharification of ozonated sugarcane bagasse (SCB) by enzymes from Myceliophthora thermophila JCP 1-4 was studied. Fungal enzymes provided slightly higher sugar release than commercial enzymes, working at 50 degrees C. Sugar release increased with temperature increase. Kinetic studies showed remarkable glucose release (4.99 g/L, 3% w/w dry matter) at 60 degrees C, 8 h of hydrolysis, using an enzyme load of 10 FPU (filter paper unit). FPase and beta-glucosidase activities increased during saccharification (284% and 270%, respectively). No further significant improvement on glucose release was observed increasing the enzyme load above 7.5 FPU per g of cellulose. Higher dry matter contents increased sugars release, but not yields. The fermentation of hydrolysates by Saccharomyces cerevisiae provided glucose-to-ethanol conversions around to 63%. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/19554-0 - Prospecting of thermophilic filamentous fungi producers of cellulases and application of the enzymes in sugarcane bagasse saccharification
Grantee:Daniela Alonso Bocchini
Support type: Regular Research Grants
FAPESP's process: 11/12794-6 - Isolation and selection of thermophilic/thermotolerant filamentous fungi producer of cellulases and xylanases and application of the enzymatic extracts in sugarcane bagasse saccharification
Grantee:Josiani de Cassia Pereira
Support type: Scholarships in Brazil - Master