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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Paracoccidioides brasiliensis induces recruitment of alpha 3 and alpha 5 integrins into epithelial cell membrane rafts, leading to cytokine secretion

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Barros, Bianca C. S. C. [1] ; Maza, Paloma K. [1] ; Alcantara, Cristiane [1] ; Suzuki, Erika [1]
Total Authors: 4
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Rua Botucatu 862, 6 Andar, BR-04023062 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Microbes and Infection; v. 18, n. 1, p. 68-77, JAN 2016.
Web of Science Citations: 2

Paracoccidioides brasiliensis is one of the etiological agents of paracoccidioidomycosis, a human systemic mycosis, highly prevalent in Latin America. In the present work, we demonstrate that P. brasiliensis yeasts promote IL-6 and IL-8 secretion by the human lung epithelial cell line A549 in an integrin-dependent manner. In fact, small interfering RNA directed to alpha 3 and alpha 5 integrins decreased IL-6 and IL-8 levels in P. brasiliensis-infected A549 cell cultures. This fungus also led to an increase in the expression of alpha 3 and alpha 5 integrins in this epithelial cell line. In addition, P. brasiliensis yeasts promoted alpha 3 and alpha 5 integrins clustering into A549 cell membrane rafts. Furthermore, epithelial cell membrane raft disruption with nystatin decreased IL-6 and IL-8 levels in P. brasiliensis-A549 cell cultures. Therefore, by increasing host alpha 3 and alpha 5 integrins levels and clustering these receptors into membrane rafts, P. brasiliensis yeasts may modulate host inflammation. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 11/22773-6 - Studies of the mechanisms of adhesion and cytokine modulation during interaction of epithelial cells and pathogenic fungi
Grantee:Erika Suzuki de Toledo
Support Opportunities: Regular Research Grants