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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteolytic hydrolysis of cowpea proteins is able to release peptides with hypocholesterolemic activity

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Author(s):
Marques, Marcelo Rodrigues [1] ; Fontanari, Gustavo Guadagnucci [1] ; Pimenta, Daniel Carvalho [2] ; Soares-Freitas, Rosana Manolio [1] ; Gomes Areas, Jose Alfredo [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Lab Food Funct Properties, Dept Nutr, Sch Publ Hlth, Sao Paulo, SP - Brazil
[2] Inst Butantan, Biochem & Biophys Lab, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Food Research International; v. 77, n. 1, SI, p. 43-48, NOV 2015.
Web of Science Citations: 15
Abstract

This study aimed to assess the hypocholesterolemic activity of peptides obtained by in vitro simulated human digestion of cowpea bean proteins; moreover, we have screened the bioactive peptides through chromatographic separation by RP-HPLC of the 3 kDa molecular mass cut-off fraction of hydrolyzed isolated cowpea protein. Micellar solubility of cholesterol was measured after adding 35 mu g mL(-1) of each fraction on in vitro prepared intestine-like micelles. The inhibiting activity of each fraction (50 mu g mL(-1)) also was tested on the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase). The whole hydrolysate was analyzed by `de novo' mass spectrometry peptide sequencing (RP-HPLC-MS2) and the top score candidate peptide sequences were further analyzed by computational modeling. All collected fractions inhibited the initial HMGCoA reductase activity by 47.8 to 57.1%. They also reduced cholesterol micellar solubilization, with fraction 1 being the most effective (71.7%). The peptide conserved domains may interact with the phosphatidylcholine added in the reaction of the cholesterol micelles. According with a computational prediction, the only peptide able to bind significantly the HMG-CoA reductase was GCTLN. This is the first report of peptide fractions from cowpea bean protein released by human digestion enzymes (pepsin followed by pancreatin) assigned to its cholesterol-lowering effect. These routes may define their action in lipid metabolism. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/15900-4 - Bioavailability of peptides from proteins of lupine, cowpea and amaranth, by permeability through Caco-2 cells and alterations in gene expression of the transporters associated with intestinal cholesterol absorption
Grantee:José Alfredo Gomes Arêas
Support Opportunities: Regular Research Grants
FAPESP's process: 11/04179-0 - Identification of hypocholesterolemic peptides from extruded cowpea beans (Vigna unguiculata L. Walp)
Grantee:Marcelo Rodrigues Marques
Support Opportunities: Scholarships in Brazil - Master