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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis

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Author(s):
Ayo, Christiane Maria [1, 2] ; da Silveira Camargo, Ana Vitoria [1, 2] ; Frederico, Fabio Batista [3, 1] ; Siqueira, Rubens Camargo [2] ; Previato, Mariana [2, 1] ; Antunes Murata, Fernando Henrique [2, 1] ; Silveira-Carvalho, Aparecida Perpetuo [2] ; Barbosa, Amanda Pires [3, 1] ; Brandao de Mattos, Cinara de Cassia [1, 2] ; de Mattos, Luiz Carlos [2, 1]
Total Authors: 10
Affiliation:
[1] Fundacao Fac Reg Med Sao Jose Do Rio Preto, FAMERP Toxoplasma Res Grp, Sao Jose Do Rio Preto, SP - Brazil
[2] Fac Med Sao Jose Rio Preto Sao Jose Rio Preto, Dept Mol Biol, Immunogenet Lab, Sao Jose Do Rio Preto, SP - Brazil
[3] Hosp Base Sao Jose Rio Preto, Ophthalmol Outpatient Clin, Fundacao Fac Reg Med Sao Jose Rio Preto, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 10, n. 12 DEC 16 2015.
Web of Science Citations: 3
Abstract

This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of the ocular manifestation observed: primary (n = 120) or recurrent (n = 28). Genotyping of the MICA and HLA alleles was performed by the polymerase chain reaction-sequence specific oligonucleotide technique (PCR-SSO; One Lambda (R)) and the MICA-129 polymorphism (rs1051792) was identified by nested polymerase chain reaction (PCR-RFLP). Significant associations involving MICA polymorphisms were not found. Although the MICA{*}002 similar to HLA-B{*}35 haplotype was associated with increased risk of developing ocular toxoplasmosis (P-value = 0.04; OR = 2.20; 95% CI = 1.05-4.60), and the MICA{*}008 similar to HLA-C{*}07 haplotype was associated with protection against the development of manifestations of ocular toxoplasmosis (P-value = 0.009; OR: 0.44; 95% CI: 0.22-0.76), these associations were not statistically significant after adjusting for multiple comparisons. MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population. (AU)

FAPESP's process: 13/06580-9 - KIR genes, their HLA ligands and MICA gene polymorphism in ocular toxoplasmosis and in digestive form of Chagas disease
Grantee:Christiane Maria Ayo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/13723-6 - MHC class I chain-related gene A polymorphisms and linkage disequilibrium with HLAB and HLA-C alleles in ocular toxoplasmosis
Grantee:Cinara de Cássia Brandão
Support type: Regular Research Grants - Publications - Scientific article
FAPESP's process: 13/15879-8 - Toxoplasma gondii infection: serological and molecular characterization in patients attended in public health service (SUS)
Grantee:Fernando Henrique Antunes Murata
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/17540-2 - Histo blood groups like risk factor for ocular toxoplasmosis
Grantee:Luiz Carlos de Mattos
Support type: Regular Research Grants
FAPESP's process: 13/10050-5 - Serological, molecular and images in patients with active lesions or acute relapse of ocular infection by Toxoplasma gondii: a study follow-up
Grantee:Mariana Previato
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/25650-8 - Class II HLA genes (DRB1 and DQB1) as risck factors for ocular toxoplasmosis
Grantee:Luiz Carlos de Mattos
Support type: Regular Research Grants