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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of low contents of superhydrophilic MWCNT on the properties and cell viability of electrospun poly (butylene adipate-co-terephthalate) fibers

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Rodrigues, Bruno V. M. [1] ; Silva, Aline S. [1] ; Melo, Gabriela F. S. [2] ; Vasconscellos, Luana M. R. [2] ; Marciano, Fernanda R. [1] ; Lobo, Anderson O. [1]
Total Authors: 6
[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Biomed Nanotechnol, Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 59, p. 782-791, FEB 1 2016.
Web of Science Citations: 27

The use of poly (butylene adipate-co-terephthalate) (PBAT) in tissue engineering, more specifically in bone regeneration, has been underexplored to date due to its poor mechanical resistance. In order to overcome this drawback, this investigation presents an approach into the preparation of electrospun nanocomposite fibers from PBAT and low contents of superhydrophilic multi-walled carbon nanotubes (sMWCNT) (0.1-0.5 wt.%) as reinforcing agent. We employed a wide range of characterization techniques to evaluate the properties of the resulting electrospun nanocomposites, including Field Emission Scanning Electronic Microscopy (FE-SEM), Transmission Electronic Microscopy (TEM), tensile tests, contact angle measurements (CA) and biological assays. FE-SEM micrographs showed that while the addition of sMWCNT increased the presence of beads on the electrospun fibers' surfaces, the increase of the neat charge density due to their presence reduced the fibers' average diameter. The tensile test results pointed that sMWCNT acted as reinforcement in the PBAT electrospun matrix, enhancing its tensile strength (from 13 to 3.6 MPa with addition of 0.5 wt.% of sMWCNT) and leading to stiffer materials (lower elongation at break). An evaluation using MG63 cells revealed cell attachment into the biomaterials and that all samples were viable for biomedical applications, once no cytotoxic effect was observed. MG-63 cells osteogenic differentiation, measured by ALP activity, showed that mineralized nodules formation was increased in PBAT/0.5%CNTs when compared to control group (cells). This investigation demonstrated a feasible novel approach for producing electrospun nanocomposites from PBAT and sMWCNT with enhanced mechanical properties and adequate cell viability levels, which allows for a wide range of biomedical applications for these materials. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 15/01259-3 - Electrospinning by solution of PBAT built nanohidroxiapatita
Grantee:Aline dos Santos Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers
Grantee:Bruno Vinícius Manzolli Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Research Grants - Young Investigators Grants