| Full text | |
| Author(s): |
Corral, Marcelo Andreetta
[1, 2]
;
de Paula, Fabiana Martins
[1, 2]
;
Gottardi, Maiara
[1, 2]
;
Correia Lima Meisel, Dirce Mary
[1, 2]
;
Pagliusi Castilho, Vera Lucia
[3]
;
do Nascimento Goncalves, Elenice Messias
[3]
;
Chieffi, Pedro Paulo
[1, 4]
;
Borges Gryschek, Ronaldo Cesar
[1, 2]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-05403000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, Hosp Clin, Dept Molestias Infecciosas & Parasitarias LIM 06, BR-05403000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med, Secao Parasitol, Div Lab Cent, Hosp Clin, BR-05403000 Sao Paulo, SP - Brazil
[4] Santa Casa Misericordia Sao Paulo, Fac Ciencias Med, Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 57, n. 5, p. 427-430, SEP-OCT 2015. |
| Web of Science Citations: | 4 |
| Abstract | |
SUMMARY The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis. (AU) | |
| FAPESP's process: | 13/04236-9 - Immunoproteomics applied to the diagnosis human strongyloidiasis used antigen Strongyloides venezuelensis |
| Grantee: | Ronaldo Cesar Borges Cryschek |
| Support Opportunities: | Regular Research Grants |