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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells

Full text
Author(s):
Franca, M. M. [1] ; Abreu, N. P. [1] ; Vrechi, T. A. M. [1] ; Lotfi, C. F. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, BR-05508 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 48, n. 12, p. 1087-1094, DEC 2015.
Web of Science Citations: 6
Abstract

During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1 overexpression, following the transfection of pCMVMycPod-1, significantly decreased the endogenous levels of Sf-1 mRNA and protein in F/R cells, but not in G cells, and also decreased the expression of the SF-1 target StAR in F/R cells. In G cells overexpressing POD-1, no modulation of the expression of SF-1 targets, StAR and CYP11B2, was observed. Our data showing that G and F/R cells respond differently to ectopic POD-1 expression emphasize the functional differences between the outer and inner zones of the adrenal cortex, and support the hypothesis that SF-1 is regulated by POD-1/Tcf21 in normal adrenocortical cells lacking the alterations in cellular physiology found in tumor cells. (AU)

FAPESP's process: 12/19512-9 - Analysis of signaling pathways induced by NH2-terminal peptides of POMC (N-POMC) in primary cultures of rat adrenal
Grantee:Talita Aparecida de Moraes Vrechi
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/00771-9 - The role and mechanism of the Sf-1 and Lrh-1 transcription factors regulation in the cell culture of humam adrenal tumor
Grantee:Monica Malheiros França
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/21839-6 - Study of cell cycle of adrenocortical tumor cells: analysis of oncogenes and tumor suppressor genes expression, and proliferation through the chemotherapeutic drugs action
Grantee:Claudimara Ferini Pacicco Lotfi
Support Opportunities: Regular Research Grants
FAPESP's process: 11/01031-1 - Study expression of transcription factor SF-1 and its regulation in primary cultures of rat adrenal: the role of Pod-1/TCF-21
Grantee:Nayara Pereira de Abreu
Support Opportunities: Scholarships in Brazil - Master