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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose

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Vieira Nogueira, Karoline Maria [1] ; Costa, Mariana do Nascimento [1] ; de Paula, Renato Graciano [1] ; Mendonca-Natividade, Flavia Costa [2] ; Ricci-Azevedo, Rafael [2] ; Silva, Roberto Nascimento [1]
Total Authors: 6
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell Biol & Mol & Pathogen Bioagents, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BMC Microbiology; v. 15, SEP 30 2015.
Web of Science Citations: 12

Background: The signaling second messenger cyclic AMP (cAMP) regulates many aspects of cellular function in all organisms. Previous studies have suggested a role for cAMP in the regulation of gene expression of cellulolytic enzymes in Trichoderma reesei (anamorph of Hypocrea jecorina). Methods: The effects of cAMP in T. reesei were analyzed through both activity and expression of cellulase, intracellular cAMP level measurement, western blotting, indirect immunofluorescence and confocal microscopy. Results: To elucidate the involvement of cAMP in the cellulase expression, we analyzed the growth of the mutant strain Delta acy1 and its parental strain QM9414 in the presence of the inducers cellulose, cellobiose, lactose, or sophorose, and the repressor glucose. Our results indicated that cAMP regulates the expression of cellulase in a carbon source-dependent manner. The expression cel7a, and cel6a genes was higher in the presence of sophorose than in the presence of cellulose, lactose, cellobiose, or glucose. Moreover, intracellular levels of cAMP were up to four times higher in the presence of sophorose compared to other carbon sources. Concomitantly, our immunofluorescence microscopy and western blot data suggest that in the presence of sophorose, cAMP may regulate secretion of cellulolytic enzymes in T. reesei. Conclusions: These results allow us to better understand the role of cAMP and expand our knowledge on the signal transduction pathways involved in the regulation of cellulase expression in T. reesei. Finally, our data may help develop new strategies to improve the expression of cel7a and cel6a genes, and therefore, favor their application in several biotechnology fields. (AU)

FAPESP's process: 14/23653-2 - Characterization of new sugar transporters involved in the regulation of lignocellulosic biomass degradation of in Trichoderma reesei
Grantee:Roberto do Nascimento Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 13/03681-9 - Performance of casein kinase II in carbon catabolite repression (CCR) in Trichoderma reesei (Hypocrea jecorina)
Grantee:Karoline Maria Vieira Nogueira
Support Opportunities: Scholarships in Brazil - Master