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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of tyrosol on adhesion of Candida albicans and Candida glabrata to acrylic surfaces

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Monteiro, Douglas Roberto [1] ; Feresin, Leonardo Perina [1] ; Arias, Lais Salomao [1] ; Ricardo Barao, Valentim Adelino [2] ; Barbosa, Debora Barros [3] ; Botazzo Delbem, Alberto Carlos [1]
Total Authors: 6
[1] Univ Estadual Paulista, UNESP, Dept Pediat Dent & Publ Hlth, Aracatuba Dent Sch, BR-16015050 Aracatuba Sao Paulo - Brazil
[2] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, BR-13414903 Piracicaba Sao Paulo - Brazil
[3] Univ Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, BR-16015050 Aracatuba Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Medical Mycology; v. 53, n. 7, p. 656-665, SEP 2015.
Web of Science Citations: 15

The prevention of adhesion of Candida cells to acrylic surfaces can be regarded as an alternative to prevent denture stomatitis. The use of quorum sensing molecules, such as tyrosol, could potentially interfere with the adhesion process. Therefore, the aim of this study was to assess the effect of tyrosol on adhesion of single and mixed cultures of Candida albicans and Candida glabrata to acrylic resin surfaces. Tyrosol was diluted in each yeast inoculum (10(7) cells/ml in artificial saliva) at 25, 50, 100, and 200 mM. Then, each dilution was added to wells of 24-well plates containing the acrylic specimens, and the plates were incubated at 37A degrees C for 2 h. After, the effect of tyrosol was determined by total biomass quantification, metabolic activity of the cells and colony-forming unit counting. Chlorhexidine gluconate (CHG) was used as a positive control. Data were analyzed using analysis of variance (ANOVA) and the Holm-Sidak post hoc test (alpha = 0.05). The results of total biomass quantification and metabolic activity revealed that the tyrosol promoted significant reductions (ranging from 22.32 to 86.16%) on single C. albicans and mixed cultures. Moreover, tyrosol at 200 mM and CHG significantly reduced (p < 0.05) the number of adhered cells to the acrylic surface for single and mixed cultures of both species, with reductions ranging from 1.74 to 3.64-log(10.) In conclusion, tyrosol has an inhibitory effect on Candida adhesion to acrylic resin, and further investigations are warranted to clarify its potential against Candida infections. (AU)

FAPESP's process: 13/17767-2 - Effect of Tyrosol on adhesion capacity of Candida cells to acrylic resin surface
Grantee:Leonardo Perina Feresin
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/10285-2 - Effect of tyrosol on virulence factors of Candida albicans, Candida glabrata and Streptococcus mutans
Grantee:Alberto Carlos Botazzo Delbem
Support type: Regular Research Grants