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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of different carbohydrate sources on fructan metabolism in plants of Chrysolaena obovata grown in vitro

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Author(s):
Trevisan, Flavio [1, 2] ; Oliveira, Vanessa F. [1, 3] ; Carvalho, Maria A. M. [1] ; Gaspar, Marilia [1]
Total Authors: 4
Affiliation:
[1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, BR-04045972 Sao Paulo - Brazil
[2] Inst Fed Sao Paulo, Sao Roque - Brazil
[3] Univ Mogi das Cruzes, Nucleo Ciencias Saude, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 6, SEP 7 2015.
Web of Science Citations: 4
Abstract

Chrysolaena obovata (Less.) Dematt., previously named Vernonia herbacea, is an Asteraceae native to the Cerrado which accumulates about 80% of the rhizophore dry mass as inulin-type fructans. Considering its high inulin production and the wide application of fructans, a protocol for C. obovata in vitro culture was recently established. Carbohydrates are essential for in vitro growth and development of plants and can also act as signaling molecules involved in cellular adjustments and metabolic regulation. This work aimed to evaluate the effect of different sources of carbohydrate on fructan metabolism in plants grown in vitro. For this purpose, C. obovata plants cultivated in vitro were submitted to carbon deprivation and transferred to MS medium supplemented with sucrose, glucose or fructose. Following, their fructan composition and activity and expression of genes encoding enzymes for fructan synthesis (1 -SST and 1 -FFT) and degradation (1-FEH) were evaluated. For qRT-PCR analysis partial cDNA sequences corresponding to two different C. obovata genes, 1-SST and 1-FFT, were isolated. As expected, C. obovata sequences showed highest sequence identity to other Asteraceae 1 -SST and 1 -FFT, than to Poaceae related proteins. A carbon deficit treatment stimulated the transcription of the gene 1-FEH and inhibited 1-SST and 1 -FFT and carbohydrate supplementation promoted reversal of the expression profile of these genes. With the exception of 1 -FFT, a positive correlation between enzyme activity and gene expression was observed. The overall results indicate that sucrose, fructose and glucose act similarly on fructan metabolism and that 1 -FEH and 1 -SST are transcriptionally regulated by sugar in this species. Cultivation of plants in increasing sucrose concentrations stimulated synthesis and inhibited fructan mobilization, and induced a distinct pattern of enzyme activity for 1 -SST and 1 -FFT, indicating the existence of a mechanism for differential regulation between them. (AU)

FAPESP's process: 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers
Grantee:Marcia Regina Braga
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/16688-6 - In vitro regeneration of Vernonia herbacea (Vell.) Rusby and the activity and expression of the fructan metabolizing enzymes
Grantee:Flavio Trevisan
Support type: Scholarships in Brazil - Doctorate