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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Candida-streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes

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Author(s):
Bertolini, M. M. [1] ; Xu, H. [1] ; Sobue, T. [1] ; Nobile, C. J. [2] ; Del Bel Cury, A. A. [3] ; Dongari-Bagtzoglou, A. [1]
Total Authors: 6
Affiliation:
[1] Univ Connecticut, Sch Dent Med, Div Periodontol, Farmington, CT 06030 - USA
[2] Univ Calif, Sch Nat Sci, Merced, CA - USA
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Div Prosthodont & Periodontol, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecular Oral Microbiology; v. 30, n. 4, p. 307-322, AUG 2015.
Web of Science Citations: 18
Abstract

Candida albicans and streptococci of the mitis group form communities in multiple oral sites, where moisture and nutrient availability can change spatially or temporally. This study evaluated structural and virulence characteristics of Candida-streptococcal biofilms formed on moist or semidry mucosal surfaces, and tested the effects of nutrient availability and hyphal morphotype on dual-species biofilms. Three-dimensional models of the oral mucosa formed by immortalized keratinocytes on a fibroblast-embedded collagenous matrix were used. Infections were carried out using Streptococcus oralis strain 34, in combination with a C.albicans wild-type strain, or pseudohyphal-forming mutant strains. Increased moisture promoted a homogeneous surface biofilm by C.albicans. Dual biofilms had a stratified structure, with streptococci growing in close contact with the mucosa and fungi growing on the bacterial surface. Under semidry conditions, Candida formed localized foci of dense growth, which promoted focal growth of streptococci in mixed biofilms. Candida biofilm biovolume was greater under moist conditions, albeit with minimal tissue invasion, compared with semidry conditions. Supplementing the infection medium with nutrients under semidry conditions intensified growth, biofilm biovolume and tissue invasion/damage, without changing biofilm structure. Under these conditions, the pseudohyphal mutants and S.oralis formed defective superficial biofilms, with most bacteria in contact with the epithelial surface, below a pseudohyphal mass, resembling biofilms growing in a moist environment. The presence of S.oralis promoted fungal invasion and tissue damage under all conditions. We conclude that moisture, nutrient availability, hyphal morphotype and the presence of commensal bacteria influence the architecture and virulence characteristics of mucosal fungal biofilms. (AU)

FAPESP's process: 12/21011-8 - Growth conditions influence at structural and virulence characteristics of Candida and Streptococcus biofilms developed on in vitro models of human oral mucosa
Grantee:Martinna de Mendonça e Bertolini
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/00274-3 - Influence of fluconazole in immune response to infection by Candida albicans on in vitro model of oral epithelium
Grantee:Altair Antoninha Del Bel Cury
Support type: Regular Research Grants