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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence of Mycoplasma genitalium and Mycoplasma hominis in urogenital tract of Brazilian women

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Campos, Guilherme Barreto [1] ; Lobao, Tassia Neves [1] ; Selis, Nathan Neves [2] ; Amorim, Aline Teixeira [1] ; Martins, Hellen Braga [2] ; Barbosa, Maysa Santos [2] ; Caldeira Oliveira, Thiago Henrique [2] ; dos Santos, Djanilson Barbosa [3] ; Figueiredo, Tiana Baqueiro [2] ; Marques, Lucas Miranda [1, 2] ; Timenetsky, Jorge [1]
Total Authors: 11
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Bahia, Inst Multidisciplinar Saude, BR-45055090 Vitoria Da Conquista, BA - Brazil
[3] Univ Fed Reconcavo Baiano, Ctr Ciencias Saude, BR-44570000 Santo Antonio De Jesus, BA - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BMC INFECTIOUS DISEASES; v. 15, FEB 14 2015.
Web of Science Citations: 10

Background: The role of Mycoplasma hominis and M. genitalium in urogenital tract infections remains unknown. Furthermore these mollicutes present a complex relationship with the host immune response. The role of inflammatory cytokines in infections also makes them good candidates to investigate bacterial vaginosis and mycoplasma genital infections. Therefore, the aim of this study was to detect the above-mentioned mollicutes by quantitative Polymerase Chain Reaction (qPCR) methodologies in vaginal swabs and dosage of cytokines. Methods: Vaginal swabs and peripheral blood were collected from 302 women, including healthy individuals. The molecular findings were correlated with some individual behavioral variables, clinical and demographic characteristics, presence of other important microorganisms in vaginal swabs, and levels of interleukin (IL)-1 beta and IL-6. Results: M. hominis and M. genitalium were detected in 31.8% and 28.1% of samples, respectively. The qPCR results were associated with clinical signs and symptoms of the infections studied. The frequency of Trichomonas vaginalis, Gardnerella vaginalis, Neisseria gonorrhoeae and Chlamydia trachomatis was 3.0%, 21.5%, 42.4%, and 1.7% respectively. Increased levels of IL-1 beta were associated with the presence of M. hominis and signs and/or symptoms of the genital infection of women studied. Conclusion: IL-1 beta production was associated with the detection of M. hominis by qPCR. The sexual behavior of women studied was associated with the detection of mycoplasma and other agents of genital infections. (AU)

FAPESP's process: 11/10138-4 - Detection of mollicutes in the female urogenital tract and its relation to genetic polymorphisms and expression of cytokines in women of Vitoria da Conquista, BA
Grantee:Jorge Timenetsky
Support Opportunities: Regular Research Grants