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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Surface Characterization and Osteoblast-like Cells Culture on Collagen Modified PLDLA Scaffolds

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Mas, Bruna Antunes [1, 2] ; de Mello Cattani, Silvia Mara [2] ; Cipriano Rangel, Rita de Cassia [3] ; Ribeiro, Gabrielle de Almeida [4] ; Cruz, Nilson Cristino [3] ; Leite, Fabio de Lima [4] ; de Paula Nascente, Pedro Augusto [5] ; de Rezende Duek, Eliana Aparecida [1, 2]
Total Authors: 8
[1] Univ Estadual Campinas UNICAMP, Fac Mech Engn, Dept Mat Engn, BR-13083970 Campinas, SP - Brazil
[2] Pontificia Univ Catolica Sao Paulo PUC SP, Lab Biomat, BR-18030070 Sorocaba, SP - Brazil
[3] Univ Estadual Paulista UNESP, Lab Technol Plasmas, BR-18085180 Sorocaba, SP - Brazil
[4] Univ Fed Sao Carlos UFSCar, Dept Mat Engn, BR-18052780 Sorocaba, SP - Brazil
[5] Univ Fed Sao Carlos UFSCar, Dept Mat Engn, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 6

Surface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to stimulate bone regeneration by a simple and low-cost method of surface biofunctionalization of the poly (L-co-D, L lactic acid)-PLDLA scaffolds. The method was obtained by grafting reaction of carboxyl groups (-COOH) on their surface via acrylic acid (AAc) polymerization process, followed by immobilization of collagen type I (Col). Such approach resulted in a surface morphology markedly modified after treatment, with increase of pores and roughness on PLDLA-AAc surfaces and a network of fibrillar collagen deposition in nonspecific areas of PLDLA-Col surfaces. The cytocompatibility of collagen-immobilized scaffolds was significantly improved in terms of cellular adhesion, proliferation, collagen synthesis and maintenance of osteoblast-like phenotype, indicating, therefore, the fundamental role of collagen protein over the biological interactions that occur by bio-recognition mimetic mechanisms at biomaterials interface. These results indicate that the surface modification method used here may be useful as a strategy to develop biofunctional scaffolds, which provide a more successful clinical application of biomaterials in the tissue engineering field. (AU)

FAPESP's process: 07/05089-9 - Development of nanobiosensors using atomic force spectroscopy: application for detecting pesticides
Grantee:Fabio de Lima Leite
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/12081-2 - Collagen imobilization on poly (L-co-D, L lactic acid) scaffolds
Grantee:Eliana Aparecida de Rezende Duek
Support Opportunities: Regular Research Grants