| Full text | |
| Author(s): |
Corral, Marcelo Andreetta
[1]
;
Paula, Fabiana Martins
[2]
;
Gottardi, Maiara
[1]
;
Correia Lima Meisel, Dirce Mary
[2]
;
Chieffi, Pedro Paulo
[1, 2, 3]
;
Borges Gryschek, Ronaldo Cesar
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Trop Med Sao Paulo, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Fac Med, Lab Invest Med LIM 06, Hosp Clin, BR-05403000 Sao Paulo - Brazil
[3] Fac Ciencias Med, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Revista do Instituto de Medicina Tropical de São Paulo; v. 57, n. 1, p. 77-80, JAN-FEB 2015. |
| Web of Science Citations: | 5 |
| Abstract | |
Strongyloides venezuelensis is a parasitic nematode of rodents frequently used to obtain heterologous antigens for the immunological diagnosis of human strongyloidiasis. The aim of this study was to evaluate membrane fractions from S. venezuelensis for human strongyloidiasis immunodiagnosis. Soluble and membrane fractions were obtained in phosphate saline (SS and SM) and Tris-HCl (TS and TM) from filariform larvae of S. venezuelensis. Ninety-two serum samples (n = 92) were obtained from 20 strongyloidiasis patients (Group I), 32 from patients with other parasitic diseases (Group II), and 40 from healthy individuals (Group III), and were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions (SS and TS) showed 90.0% sensitivity and 88.9% specificity, whereas the membrane fractions (SM and TM) showed 95.0% sensitivity and 94.4% specificity. The present results suggest the possible use of membrane fractions of S. venezuelensis as an alternative antigen for human strongyloidiasis immunodiagnosis. (AU) | |
| FAPESP's process: | 13/04236-9 - Immunoproteomics applied to the diagnosis human strongyloidiasis used antigen Strongyloides venezuelensis |
| Grantee: | Ronaldo Cesar Borges Cryschek |
| Support Opportunities: | Regular Research Grants |