Silva, Felipe A.
Lima, Lionete N.
Masunaga, Sueli H.
Tardioli, Paulo W.
Giordano, Roberto C.
Araujo-Moreira, Fernando M.
Giordano, Raquel L. C.
Total Authors: 8
 Univ Fed Sao Carlos UFScar, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
 Montana State Univ, Dept Phys, Bozeman, MT 59717 - USA
 Univ Fed Sao Carlos UFScar, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
 Univ Fed Sao Carlos UFScar, Dept Phys, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 4
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS;
Web of Science Citations:
Magnetic carriers are an effective option to withdraw selected target molecules from complex mixtures or to immobilize enzymes. This paper describes the synthesis of robust silica magnetic microparticles (SMMps), particularly designed for applications in bioprocesses. SMMps were synthesized in a micro-emulsion, using sodium silicate as the silica source and superparamagnetic iron oxide nanoparticles as the magnetic core. Thermally resistant particles, with high and accessible surface area, narrow particle size distribution, high saturation magnetization, and with superparamagnetic properties were obtained. Several reaction conditions were tested, yielding materials with saturation magnetization between 45 and 63 emu g(-1), particle size between 2 and 200 mu m and average diameter between 11.2 and 15.9 mu m, surface area between 49 and 103 m(2) g(-1) and pore diameter between 2 and 60 nm. The performance of SMMps in a bioprocess was evaluated by the immobilization of Pseudomonas fluorescens lipase on to octyl modified SMMp, the biocatalyst obtained was used in the production of butyl butyrate with good results. (C) 2014 Elsevier B.V. All rights reserved. (AU)