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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Nanobiosensor Based on 4-Hydroxyphenylpyruvate Dioxygenase Enzyme for Mesotrione Detection

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Garcia, Pamela Soto [1] ; Dario Moreau, Alberto Luis [1, 2] ; Magalhaes Ierich, Jessica Cristiane [1] ; Araujo Vig, Ana Carolina [1] ; Higa, Akemi Martins [1] ; Oliveira, Guedmiller S. [1] ; Abdalla, Fabio Camargo [3] ; Hausen, Moema [1] ; Leite, Fabio L. [1]
Total Authors: 9
[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Nanoneurobiophys Res Grp, BR-18052780 Sao Carlos, SP - Brazil
[2] Fed Inst Educ Sci & Technol Itapetininga, BR-18202000 Itapetininga - Brazil
[3] Univ Fed Sao Carlos, Dept Biol, Lab Struct & Funct Biol, BR-18052780 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: IEEE SENSORS JOURNAL; v. 15, n. 4, p. 2106-2113, APR 2015.
Web of Science Citations: 11

The herbicide residue from intensive agricultural activity provokes environmental disturbances and human health injuries. Among the enzymatic disruptor herbicides, mesotrione is able to inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD), which plays a key role in the carotenoid synthesis. Therefore, enzyme-based sensors are innovative options for monitoring herbicides used in agriculture. Compared to the standard sensors, biosensors have assorted advantages, such as practicality, quick response, low cost, and high sensitivity. A nanobiosensor was developed herein based on HPPD for mesotrione detection. Theoretically, the molecular docking and molecular dynamics simulation estimated the interacting regions of HPPD with mesotrione. Experimentally, the atomic force microscope tip functionalization with HPPD immobilized in self-assembled monolayers was confirmed by fluorescence microscopy and atomic force spectroscopy. The cross-linker N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride was responsible for properly preserving the enzyme on the tip. The nanobiosensor proposed here was successfully able to detect mesotrione molecules. Such effectiveness in the development of nanobiosensors promises reliable, precise, and low-cost techniques, which apply to a broad range of issues, from ecology to medicine. (AU)

FAPESP's process: 11/17840-6 - Effect of cadmium and Roundup® on internal organs of Bombus morio and Bombus atratus (Hymenoptera: Bombini)
Grantee:Fábio Camargo Abdalla
Support Opportunities: Regular Research Grants
Grantee:Guedmiller Souza de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/12082-4 - Molecular modeling of the antigen-antibody complex to the investigations of autoimmune demyelinating diseases
Grantee:Jéssica Cristiane Magalhães Ierich
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/04599-6 - Development of nanobiosensors based on Intelligent surfaces
Grantee:Aline Cristine Nanuh da Silva
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 07/05089-9 - Development of nanobiosensors using atomic force spectroscopy: application for detecting pesticides
Grantee:Fabio de Lima Leite
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 13/21958-8 - Molecular evaluation of the immunoglobulin G (IgG) using homology modeling for the application in nanobiosensors
Grantee:Ana Carolina Araujo Vig
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 08/57859-5 - Biorrational control of insect pests
Grantee:Maria Fátima das Graças Fernandes da Silva
Support Opportunities: Research Projects - Thematic Grants