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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A metabolomic protocol for plant systematics by matrix-assisted laser-desorption/ionization time-of flight mass spectrometry

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Ernst, Madeleine [1] ; Silva, Denise B. [1] ; Silva, Ricardo [2] ; Monge, Marcelo [3] ; Semir, Joao [3] ; Vencio, Ricardo Z. N. [2] ; Lopes, Norberto P. [1]
Total Authors: 7
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Chem & Phys, Nucleo Pesquisa Prod Nat & Sintet, BR-14040903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Comp & Math, FFCLRP, BR-14040903 Sao Paulo - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Analytica Chimica Acta; v. 859, p. 46-58, FEB 15 2015.
Web of Science Citations: 3

Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) has been widely used for the identification and classification of microorganisms based on their proteomic fingerprints. However, the use of MALDI-TOF MS in plant research has been very limited. In the present study, a first protocol is proposed for metabolic fingerprinting by MALDI-TOF MS using three different MALDI matrices with subsequent multivariate data analysis by in-house algorithms implemented in the R environment for the taxonomic classification of plants from different genera, families and orders. By merging the data acquired with different matrices, different ionization modes and using careful algorithms and parameter selection, we demonstrate that a close taxonomic classification can be achieved based on plant metabolic fingerprints, with 92% similarity to the taxonomic classifications found in literature. The present work therefore highlights the great potential of applying MALDI-TOF MS for the taxonomic classification of plants and, furthermore, provides a preliminary foundation for future research. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/05215-0 - Metabolomics in plant systematics: the arnica model
Grantee:Madeleine Ernst
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/18031-7 - Application of MALDI-MS/MS in the vicenin-2 and derivatives analyses: Understanding the ionization processes to production of the images
Grantee:Denise Brentan da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 10/14926-4 - Bioinformatics applied to Bioenergy: Probabilistic annotation of cane sugar the metabolome
Grantee:Ricardo Roberto da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 09/54098-6 - Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances
Grantee:Norberto Peporine Lopes
Support type: Multi-user Equipment Program