Staphylococcus aureus enterotoxins A and B inhibit... - BV FAPESP
Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Staphylococcus aureus enterotoxins A and B inhibit human and mice eosinophil chemotaxis and adhesion in vitro

Full text
Author(s):
Squebola-Cola, Dalize M. [1] ; De Mello, Glaucia C. [1] ; Anhe, Gabriel F. [1] ; Condino-Neto, Antonio [2] ; DeSouza, Ivani A. [3] ; Antunes, Edson [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, BR-13084971 Campinas, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil
[3] FMJ, Dept Biol & Physiol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Immunopharmacology; v. 23, n. 2, p. 664-671, DEC 2014.
Web of Science Citations: 1
Abstract

Staphylococcus aureus aggravates the allergic eosinophilic inflammation. We hypothesized that Staphylococcus aureus-derived enterotoxins directly affect eosinophil functions. Therefore, this study investigated the effects of Staphylococcal enterotoxins A and B (SEA and SEB) on human and mice eosinophil chemotaxis and adhesion in vitro, focusing on p38 MAPK phosphorylation and intracellular Ca2+ mobilization. Eosinophil chemotaxis was evaluated using a microchemotaxis chamber, whereas adhesion was performed in VCAM-1 and ICAM-1-coated plates. Measurement of p38 MAPK phosphorylation and intracellular Ca2+ levels were monitored by flow cytometry and fluorogenic calcium-binding dye, respectively. Prior incubation (30 to 240 min) of human blood eosinophils with SEA (0.5 to 3 ng/ml) significantly reduced eotaxin-, PAF- and RANTES-induced chemotaxis (P < 0.05). Likewise, SEB (1 ng/ml, 30 min) significantly reduced eotaxin-induced human eosinophil chemotaxis (P < 0.05). The reduction of eotaxin-induced human eosinophil chemotaxis by SEA and SEB was prevented by anti-MHC monoclonal antibody (1 mu g/ml). In addition, SEA and SEB nearly suppressed the eotaxin-induced human eosinophil adhesion in ICAM-1- and VCAM-1-coated plates. SEA and SEB prevented the increases of p38 MAPK phosphorylation and Ca2+ levels in eotaxin-activated human eosinophils. In separate protocols, we evaluated the effects of SEA on chemotaxis and adhesion of eosinophils obtained from mice bone marrow. SEA (10 ng/ml) significantly reduced the eotaxin-induced chemotaxis along with cell adhesion to both ICAM-1 and VCAM-1-coated plates (P < 0.05). In conclusion, the inhibition by SEA and SEB of eosinophil functions (chemotaxis and adhesion) are associated with reductions of p38 MAPK phosphorylation and intracellular Ca2+ mobilization. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/10869-6 - Modulation of mice pulmonary allergic responses by the sthaphylococcal enterotoxin types A and B (SEA and SEB)and human eosinophil.
Grantee:Dalize Maria Squebola Cola
Support Opportunities: Scholarships in Brazil - Doctorate