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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of antimicrobial agents incorporated into resilient denture relines on the Candida albicans biofilm

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Bueno, M. G. [1] ; Urban, V. M. [2] ; Barberio, G. S. [1] ; da Silva, W. J. [3] ; Porto, V. C. [1] ; Pinto, L. de R. [4] ; Neppelenbroek, K. H. [1]
Total Authors: 7
[1] Univ Sao Paulo, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil
[2] Univ Estadual Ponta Grossa, Dept Dent, Ponta Grossa - Brazil
[3] Univ Estadual Campinas, Dent Sch Piracicaba, Piracicaba - Brazil
[4] Univ Fed Pelotas, Pelotas Sch Dent, Pelotas - Brazil
Total Affiliations: 4
Document type: Journal article
Source: ORAL DISEASES; v. 21, n. 1, p. 57-65, JAN 2015.
Web of Science Citations: 22

ObjectiveThe antimicrobial action of five drugs incorporated in temporary denture relines on the fungal biofilm was evaluated. Materials and MethodsA Candida albicans biofilm (SC5314) was formed on specimens (10x1mm) of materials (Trusoft and Softone) modified or not (control) by the drugs (nystatin, miconazole, ketoconazole, itraconazole, and chlorhexidine diacetate). Cell viability was determined spectrophotometrically by the tetrazolium salt reduction assay (XTT) after 24h, 48h, and 7 and 14days of incubation. The minimum inhibitory concentrations (MICs) were those which inhibited 90% or more of fungal growth. Fungal susceptibility was confirmed by confocal laser scanning microscopy analysis. ResultsThe MICs of drugs incorporated in the materials were 0.032, 0.256, 0.128, 0.256, and 0.064gml(-1) for nystatin, miconazole, ketoconazole, itraconazole, and chlorhexidine, respectively. Images from nystatin, chlorhexidine, and ketoconazole demonstrated no viable cells. ConclusionsThe antimicrobials incorporated in the resilient materials inhibited fungal growth during 14days, with lower MICs for nystatin and chlorhexidine. (AU)

FAPESP's process: 09/04890-5 - Microbiological, physical and mechanical evaluation of resilient materials modified by the addition of antimicrobial agents for denture stomatitis treatment
Grantee:Mírian Galvão Bueno
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 10/07932-8 - Microbiological, structural, mechanical and physical evaluation of resilient materials modified by the addition of antimicrobial agents for denture stomatitis treatment
Grantee:Karin Hermana Neppelenbroek
Support Opportunities: Regular Research Grants
FAPESP's process: 09/18592-6 - Microbiological evaluation of resilient materials modified by the addition of antimicrobials for denture stomatitis treatment
Grantee:Gabriel Salles Barbério
Support Opportunities: Scholarships in Brazil - Scientific Initiation