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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response

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Author(s):
Lu, Stephen [1] ; Soares, Tatiane S. [1] ; Vaz Junior, Itabajara S. [2] ; Lovato, Diogo V. [1] ; Tanaka, Aparecida S. [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04044020 Sao Paulo - Brazil
[2] Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande do Sul, Porto Alegre, RS - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Biochimie; v. 106, p. 17-23, NOV 2014.
Web of Science Citations: 7
Abstract

The Rhipicephalus microplus tick is responsible for losses in the livestock production estimated in 2 billions USD. Despite its economical importance the knowledge in tick's physiology is sparse. In order to contribute to this scenario we describe the characterization of a cysteine proteinase inhibitor named Rmcystatin-3. Purified recombinant Rmcystatin-3 was able to inhibit cathepsin L (Ki = 2.5 nM), BmCl1 (Ki = 1.8 nM) and cathepsin B (Ki = 136 nM). Western blot and quantitative PCR analysis revealed the presence of Rmcystatin-3 in fat body, salivary gland but mainly in hemocytes. The mRNA levels of Rmcystatin-3 during bacterial challenge are drastically down-regulated. In order to define the Rmcystatin-3 possible role in tick immunity, the cystatin gene was knockdown by RNA interference with and without Escherichia coil infection. Our results showed that the Rmcystatin-3 silenced group was more immune competent to control bacterial infection than the group injected with non-related dsRNA. Taking together, our data strongly suggested an important role of Rmcystatin-3 in tick immunity. (C) 2014 Elsevier B.V. and Societe francaise de biochimie et biologie Moleculaire (SFBBM). All rights reserved. (AU)

FAPESP's process: 05/03514-9 - Studies of the physiological function and biotechnological potential of protease inhibitors and anti-hemostatics in hematophagous arthropods
Grantee:Aparecida Sadae Tanaka
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/03657-8 - Inhibitor and proteases of ectoparasites: relationship of structure-function and identification of the role of these molecules in the interaction of diseases vector e their etiological agents
Grantee:Aparecida Sadae Tanaka
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 09/17589-1 - Purification and characterization of Bmcystatin-2 a cisteine protease inhibitor presents in Rhipicephalus(Boophilus) microplus tick hemocytes.
Grantee:Stephen Lu
Support Opportunities: Scholarships in Brazil - Scientific Initiation