CHLAMYDOPHILA PNEUMONIA AND INCREASED TLR4 GENE EX... - BV FAPESP
Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CHLAMYDOPHILA PNEUMONIA AND INCREASED TLR4 GENE EXPRESSION IN LEUKOCYTES ARE ASSOCIATED WITH ACUTE MYOCARDIAL INFARCTION

Full text
Author(s):
Show less -
Lima-Neto, L. G. [1, 2] ; Hirata, R. D. C. [1] ; Luchessi, A. D. [1] ; Silbiger, V. N. [1] ; Cavichioli, D. [1] ; Dos Santos, E. S. [3] ; Sousa, A. G. M. [3] ; Sprovieri, S. R. S. [4] ; De Sousa-Junior, E. B. [4] ; Dos Santos, F. C. P. [5] ; Colombo, S. [5] ; Hirata, M. H. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo - Brazil
[2] CEUMA Univ, Immunol Lab, BR-65075120 Sao Luis, MA - Brazil
[3] Inst Dante Pazzanese Cardiol, Sao Paulo - Brazil
[4] Santa Casa Sao Paulo, Sch Med Sci, Sao Paulo - Brazil
[5] Adolfo Lutz Inst, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS; v. 28, n. 3, p. 449-460, JUL-SEP 2014.
Web of Science Citations: 4
Abstract

We investigated the relationship of the positivity for Chlamydophila pneumoniae (Cpn) and Mycoplasma pneumonia (Mpn), inflammatory and metabolic markers, and mRNA expression and polymorphisms of the TLR2, TLR4, IL-6 and TNFA genes with acute myocardial infarction (AMI). Two hundred and eighteen individuals (98 AMI and 120 non-AMI) were selected at two Clinical Centers. Blood samples were drawn to extract DNA and RNA and to measure laboratory variables including anti-Cpn IgM and IgG. Cpn and Mpn genomic DNA as well as TLR2, TLR4, IL-6 and TNFA mRNA expression were evaluated by quantitative real-time PCR (qPCR). Gene polymorphisms were detected by PCR-HRM. AMI patients had higher positivity for Cpn-DNA (17.3%) than non-AMI group (6.7%, p=0.018). In addition, Cpn-DNA positivity was an independent predictor of risk for AMI (OR: 2.56, CI: 1.08 - 6.04, p=0.031). Positivity for anti-Cpn IgG and Mpn-DNA was similar between AMI and non-AMI (p>0.05). TLR4 mRNA expression was higher in AMI than non-AMI individuals (p=0.005). CD14 -260C>T, TNFA -308A>G, TLR2 c.2258G>A, TLR4 c.896A>G and TLR4 c.1196C>T variants were not associated with increased risk for AMI (p>0.05). In the AMI group, individuals carrying CD14 -260CC genotype had higher hsCRP levels than CT/TT carriers (p=0.041). These results are suggestive that Cpn-DNA positivity and increased TLR4 mRNA expression in blood leukocytes may be associated with AMI and could be useful markers to evaluate the severity and progression of the atherosclerotic disease in AMI patients. (AU)

FAPESP's process: 06/04163-8 - The inflammatory response involvement induced by Chlamydia pneumoniae and Mycoplasma pneumoniae in the acute myocardium infarction: possible mechanism by TLR
Grantee:Mario Hiroyuki Hirata
Support Opportunities: Regular Research Grants