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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bioanalytical methods for the metalloproteomics study of bovine longissimus thoracis muscle tissue with different grades of meat tenderness in the Nellore breed (Bos indicus)

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Baldassini, Welder Angelo [1] ; Braga, Camila Pereira [2] ; Loyola Chardulo, Luis Artur [1] ; Ii Vasconcelos Silva, Josineudson Augusto [1] ; Malheiros, Jessica Moraes [3] ; de Albuquerque, Lucia Galvao [3] ; Fernandes, Talita Tanaka [2] ; Padilha, Pedro de Magalhaes [2]
Total Authors: 8
[1] Sao Paulo State Univ UNESP, Coll Vet & Anim Sci, Dept Anim Breeding & Nutr, BR-18618970 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, BR-18618970 Sao Paulo - Brazil
[3] Sao Paulo State Univ UNESP, Coll Agr & Vet Sci, Dept Anim Sci, BR-18618970 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Food Chemistry; v. 169, p. 65-72, FEB 15 2015.
Web of Science Citations: 12

The work describes a metalloproteomics study of bovine muscle tissue with different grades of meat tenderness from animals of the Nellore breed (Bos indicus) based on protein separation by two-dimensional gel electrophoresis, the identification of calcium ions in protein spots by X-ray fluorescence (SR-XRF) and the characterisation of proteins by electrospray ionisation mass spectrometry. Forty (40) specimens were selected and divided into two experimental groups: animals with tough meat (TO) and animals with tender meat (TE). A third group (P) of Piedmontese breed animals (Bos taurus) was included to serve as a comparative model for the level of meat tenderness. The procedures were efficient and preserved the metal protein structure, enabling calcium detection in protein spots by SR-XRF at a given molecular weight range of 14-97 kDa. Two proteins (pyruvate kinase and albumin) were inferred to be related to the phenotypical differences in animals from the different groups. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/14850-0 - Proteins characterization µ-calpain, m-calpain and calpastatin and their relation with the quality characteristics of Nelore (Bos indicus) selected for production meat.
Grantee:Welder Angelo Baldassini
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/16118-5 - Genomic tools to genetic improvement of direct economic important traits in Nelore cattle
Grantee:Lucia Galvão de Albuquerque
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants