Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Deposition of organic-inorganic hybrid coatings over 316L surgical stainless steel and evaluation on vascular cells

Full text
Doro, F. G. [1, 2] ; Ramos, A. P. [1] ; Schneider, J. F. [3] ; Rodrigues-Filho, U. P. [4] ; Veiga, M. A. M. S. [1] ; Yano, C. L. [5] ; Negreti, A. [5] ; Krieger, M. H. [5] ; Tfouni, E. [1]
Total Authors: 9
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Fed Bahia, Inst Quim, Dept Quim Geral & Inorgan, BR-40170290 Salvador, BA - Brazil
[3] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13563120 Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13563120 Sao Carlos, SP - Brazil
[5] Univ Estadual Campinas, UNICAMP, Dept Fisiol & Biofis, BR-13081970 Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Canadian Journal of Chemistry; v. 92, n. 10, p. 987-995, OCT 2014.
Web of Science Citations: 4

Surface coating of metallic materials using the sol-gel technique is a suitable approach to obtain hybrid materials with improved properties for biomedical applications. In this study, an AISI 316L stainless steel surface was coated with ormosils prepared from tetraethylsiloxane and 3-glycidoxypropyltrimethoxysilane or polydimethylsiloxane. The characterization of structural and surface properties was performed by several techniques. Surface microstructure, morphology, and energy are dependent on organosilane type and content. Chemical stability of coatings was investigated by static immersion tests in phosphate buffer solution at 37 degrees C, and silicon leaching after 21 days was found to be in the range of similar to 200-300 mu g L-1. Mechanical adhesion was found to be within 1.0 and 3.7 N cm(-1). The interaction of the samples and materials in the cardiovascular environment was investigated through cellular behavior. Biological assays were performed with slides to avoid any cytotoxic effects on human endothelial cells (HUVEC) and rabbit arterial smooth muscle cells (RASM). No significant alterations were observed after 24 h in the viability of RASM and HUVEC cells exposed to different coatings. No increase of HUVEC or RASM migration was observed after 24 h as evaluated by transwell migration assay. The hybrid materials showed suitable properties for potential application as biomaterials in cardiovascular environment as well as for incorporation of bioactive species with the aim to prepare drug-eluting stents. (AU)

FAPESP's process: 11/07376-0 - In vitro evaluation of the vascular reestenosis prevention and reendotheliazation with new nitric oxide eluinting materials
Grantee:Marta Helena Krieger
Support Opportunities: Regular Research Grants
FAPESP's process: 10/10674-0 - Evaluation of the ability to prevent restenosis and endothelialization promotion through biological activity of new materials eluting nitric oxide
Grantee:Claudia Lumy Yano
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 06/53266-4 - Chemistry, photochemistry and biological applications of ruthenium complexes with nitric oxide and related species. From solution to materials
Grantee:Elia Tfouni
Support Opportunities: Research Projects - Thematic Grants