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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HPLC-DAD based method for the quantification of flavonoids in the hydroethanolic extract of Tonina fluviatilis Aubl. (Eriocaulaceae) and their radical scavenging activity

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de Amorim, Marcelo R. [1] ; Rinaldo, Daniel [2] ; do Amaral, Fabiano P. [3] ; Vilegas, Wagner [3] ; Magenta, Mara A. G. [4] ; Vieira, Jr., Gerardo M. [5] ; dos Santos, Lourdes C. [1]
Total Authors: 7
[1] Univ Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP - Brazil
[2] Univ Estadual Paulista, Dept Quim, BR-17033360 Bauru, SP - Brazil
[3] Univ Estadual Paulista, BR-11350000 Sao Vicente, SP - Brazil
[4] Univ Santa Cecilia, BR-11045907 Santos, SP - Brazil
[5] Univ Fed Mato Grosso, Inst Ciencias Nat Humanas & Sociais, BR-78557267 Sinop, MT - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Química Nova; v. 37, n. 7, p. 1122-U227, 2014.
Web of Science Citations: 4

This article describes the isolation and identification of flavonoids in the hydroethanolic extract of the aerial parts from Tonina fluviatilis and evaluation of their antiradical activity. A method based on HPLC-DAD was developed and validated for detecting and quantifying flavonoids in hydroethanolic extracts. The flavonoids identified and quantified in the extract were 6,7-dimethoxyquercetin-3-O-β-D-glucopyranoside (1), 6-hydroxy-7-methoxyquercetin-3-O-β-D-glucopyranoside (2), and 6-methoxyquercetin-3-O-β-D-glucopyranoside (3). The developed method presented good validation parameters, showing that the results obtained are consistent and can be used in ensuring the quantification of these constituents in the extracts. Compounds 2 and 3 showed strong antiradical activity when compared with the positive controls (quercetin and gallic acid). (AU)

FAPESP's process: 10/10676-3 - Chemical study of Tonina fluviatilis Aubl.(Eriocaulaceae)
Grantee:Marcelo Rodrigues de Amorim
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/03882-1 - Study of polar extracts of the Eriocaulaceae by coupled techniques
Grantee:Lourdes Campaner dos Santos
Support Opportunities: Regular Research Grants