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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Central and Systemic Responses to Methionine-Induced Hyperhomocysteinemia in Mice

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de Rezende, Marina Mastelaro [1] ; D'Almeida, Vania [1]
Total Authors: 2
[1] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLoS One; v. 9, n. 8 AUG 25 2014.
Web of Science Citations: 6

Hyperhomocysteinemia has been considered a risk factor for neuropsychiatric disorders, but the mechanisms involved in this process have not been completely elucidated. The aim of this study was to analyze the influence of hyperhomocysteinemia induction by methionine supplementation considering different levels and periods of exposure in mice. For this purpose, methionine supplementation at concentrations of 0.5 and 1% were administered in water to increase homocysteinemia in male C57BL/6 mice, and was maintained for 3 time periods (2, 4 and 6 months of treatment). The results from one-carbon metabolism parameters, brain-derived neurotrophic factor (BDNF) concentrations and behavioral evaluation were compared. The 0.5% supplementation was efficient in increasing plasma homocysteine levels after 2 and 6 months. The 1% supplementation, increased plasma homocysteine after 2, 4 and 6 months. Little influence was observed in cysteine and glutathione concentrations. Frontal cortex BDNF levels showed a lack of treatment influence in all periods; only the expected decrease due to increasing age was observed. Moreover, the only behavioral alteration observed using a novel object recognition task was that which was expected with increasing age. We found that responses to hyperhomocysteinemia varied based on how it was reached, and the length of toxicity. Moreover, hyperhomocysteinemia can affect the normal pattern of one carbon metabolism during age increase in mice. These findings allow the establishment of a reliable animal model for studies in this field. (AU)

FAPESP's process: 10/00075-2 - Maternal hyperhomocysteinemia and epigenetic changes in fetal programming of genes involved in Alzheimer's Disease pathogenesis
Grantee:Vânia D'Almeida
Support type: Regular Research Grants
FAPESP's process: 11/15699-4 - Oxidative stress, behavior, and epigenetic modifications in an animal model of hyperhomocysteinemia
Grantee:Marina Mastelaro de Rezende
Support type: Scholarships in Brazil - Master