Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ozone-Induced Responses in Croton floribundus Spreng. (Euphorbiaceae): Metabolic Cross-Talk between Volatile Organic Compounds and Calcium Oxalate Crystal Formation

Full text
Cardoso-Gustavson, Poliana [1] ; Bolsoni, Vanessa Palermo [2] ; de Oliveira, Debora Pinheiro [2] ; Gromboni Guaratini, Maria Tereza [2] ; Marinho Aidar, Marcos Pereira [3] ; Marabesi, Mauro Alexandre [3] ; Alves, Edenise Segala [4] ; de Souza, Silvia Ribeiro [2]
Total Authors: 8
[1] Inst Bot, Programa Posgrad Biodiversidade Vegetal & Meio Am, Sao Paulo - Brazil
[2] Inst Bot, Nucleo Pesquisa Ecol, Sao Paulo - Brazil
[3] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, Sao Paulo - Brazil
[4] Inst Bot, Nucleo Pesquisa Anat, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 9, n. 8 AUG 28 2014.
Web of Science Citations: 4

Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O-3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O-3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O-3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA) regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O-3) that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O-3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O-3. (AU)

FAPESP's process: 12/11663-8 - Atmospheric ozone action on multiple functions of volatile organic compounds from native plants
Grantee:Silvia Ribeiro de Souza
Support Opportunities: Regular Research Grants