Abstract
The goal of this project is to develop a live attenuated vaccine against Francisella noatunensis subspecies orientalis (FNO) that can be applied to Nile tilapia (Oreochromis niloticus). The project will have five specific objectives. i.) Produce at list one mutant strain of FNO deleting the wbtA, pilA, mglA, clpB, and tolC genes from a non-mutant Brazilian strain isolated in the Fish Inst…