Abstract
Genetic manipulation tools are essential for the study of gene function in anyorganism. In plants, multiple strategies can be adopted for gene inactivation, includingchemical mutagenesis, insertion of T-DNAs or transposons and site-directed mutagenesis by CRISPR/Cas9, ZFNs or TALENs. However, the generation of large-scale mutant populations and the identification of mutants of interest is…